侵染青霉菌猕猴桃生理品质与电学特性相关性研究  

Relationship between Postharvest Physiology,Quality and Electrical Properties of Kiwifruit Infected with Penicillium expansum

作　　者：罗安伟[1] 王丹[1] 梁锦 黄天姿 张璐[1] LUO Anwei;WANG Dan;LIANG Jin;HUANG Tianzi;ZHANG Lu(College of Food Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学食品与工程学院,陕西杨凌712100

出　　处：《农业机械学报》2021年第5期332-341,307,共11页Transactions of the Chinese Society for Agricultural Machinery

基　　金：陕西省重点研发计划农业领域重点项目(2018ZDXM NY 056);杨凌示范区产学研用协同创新重大项目(2018CXY 04);杨凌示范区农业科技示范推广项目(2018 GG 21)。

摘　　要：为了完善猕猴桃品质无损检测的途径,并为猕猴桃青霉病无损检测提供新方法,以红阳猕猴桃为实验材料,注射15μL菌体浓度为1×10^(6)CFU/m L的扩展青霉孢子悬浮液,对照组果实注射等体积的无菌水,对贮藏期内两组猕猴桃的品质、生理指标以及100 Hz~3.98 MHz频率范围内的相关电学参数进行测定,通过主成分分析和相关性分析筛选出特征频率和敏感电参数,建立了数学模型。结果表明:无菌水对照组和青霉侵染组果实的特征频率分别为3 956.5 k Hz和251 Hz,敏感电参数分别为等效串联电阻R_(s)和等效串联电容C_(s),其中,无菌水对照组中β-1,3-葡聚糖酶(GLU)活性与R_(s)有较高的相关性,而青霉侵染组中可滴定酸(TA)含量与C_(s)呈极显著相关(P <0.01),并分别建立了GLU活性与R_(s)、TA质量分数与C_(s)之间的数学回归方程。电学参数可以较好地反映猕猴桃品质,通过测定电学参数即可计算出GLU活性或TA含量,从而判断果实是否被青霉菌侵染。In order to improve the way of nondestructive detection of kiwifruit and provide a new method to investigate whether the kiwifruits is infected with Penicillium expansum(P.expansum),the quality,physiological indicators and the relevant electrical parameters within the frequency range of 100 Hz~3.98 MHz of Hongyang kiwifruit which were infected with 15μL 1×10^(6) CFU/mL P.expansum spore suspension in the storage period were measured,while the control group was infected with sterile water.The characteristic frequency and sensitive electrical parameters were screened out through principal component analysis and correlation analysis,and the mathematical models were further established.The results showed that the characteristic frequency of control group treated with sterile water and the inoculated group treated with P.expansum was 3956.5 kHz and 251 Hz,respectively,the sensitive electrical parameters were equivalent series resistance R s and equivalent series capacitance C_(s),respectively.As for the control group,β-1,3-glucanase(GLU)activity was highly correlated with R s,and as for inoculated group,the titratable acid TA content was significantly correlated with C(s)(P<0.01).The mathematical regression equations were also established,which indicated that the electrical parameters can better reflect the quality of kiwifruit.As long as the measurement of electrical parameters,the value of GLU activity or TA content can be calculated to determine whether the kiwifruit was infected with P.expansum.The study provided a theoretical basis for the nondestructive detection of kiwifruit penicilliosis.

关 键 词：猕猴桃 青霉菌 生理品质 电学参数 相关性分析 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]