矿物药青礞石对PTZ点燃癫痫大鼠脑组织、血浆中金属元素的影响  

Effect of Chloriti Lapis on Metal Elements in Brain and Plasma of PTZ-kindled Epileptic Rats

作　　者：刘圣金 吴露婷 马瑜璐 房方 卞勇 单晨啸 杨文国 严辉 张志杰[2] 奥·乌力吉[3] 段金廒 LIU Sheng-jin;WU Lu-ting;MAYu-lu;FANG Fang;BIANYong;SHAN Chen-xiao;YANG Wen-guo;YANHui;ZHANG Zhi-jie;AO Wu-li-ji;DUAN Jin-ao(Key Laboratory of Chinese Medicinal Resources Recycling Utilization,National Administration of Traditional Chinese Medicine,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Inner Mongolia University for Nationalities,Tongliao 028000,China)

机构地区：[1]南京中医药大学药学院,江苏省中药资源产业化过程协同创新中心,中药资源产业化与方剂创新药物国家地方联合工程研究中心,国家中医药管理局中药资源循环利用重点研究室,南京210023 [2]中国中医科学院中药研究所,北京100700 [3]内蒙古民族大学,内蒙古通辽028000

出　　处：《中国实验方剂学杂志》2021年第11期130-138,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81673566,81303178);全国矿物药资源普查项目(2019);江苏省研究生科研与实践创新计划项目(KYCX18-1608);2017年中医药公共卫生服务补助专项(财社[2017]66号)。

摘　　要：目的:研究青礞石对戊四氮(PTZ)点燃癫痫大鼠脑组织、血浆中金属元素的影响,探讨青礞石可能的效应物质基础。方法:采用PTZ点燃法建立癫痫大鼠模型,运用电感耦合等离子体质谱法(ICP-MS)和电感耦合等离子体发射光谱法(ICP-OES)测定空白组、模型组、卡马西平组(0.1 g·kg^(-1))和青礞石组(2 g·kg^(-1))大鼠脑组织、血浆中的金属元素含量,实验数据用SPSS 18.0软件进行统计学分析。结果:与空白组比较,模型组大鼠脑组织中Sr,Sb,Ba含量明显上升(P<0.05,P<0.01);Zn,Fe,Cu,K,Li,Co,Sn,Pb含量明显下降(P<0.05,P<0.01)。与模型组比较,青礞石组大鼠脑组织中Zn,Fe,K,Li,Co,As,Pb含量明显上升(P<0.05,P<0.01);Sr,Sb含量显著下降(P<0.01)。说明青礞石对大鼠脑组织中金属元素含量向正常水平的调节具有积极作用,干预效果明确,且总体效果优于卡马西平组。与空白组比较,模型组大鼠血浆中K,Sr,Cd含量明显上升(P<0.05);Li,Al,Ti,Cr含量明显下降(P<0.05)。与模型组比较,青礞石组大鼠血浆中Ca,K,Li,Al,V含量明显上升(P<0.05,P<0.01);Fe,Ti,Sr,Cd含量明显下降(P<0.05,P<0.01)。各组间金属元素相关性分析显示,大鼠脑组织中有17对元素存在显著正相关性,2对元素存在显著负相关性,大鼠血浆组织中有8对元素存在显著正相关性,6对元素存在显著负相关性。结论:以Zn,Fe,K,Li,Co,As,Pb,Sr,Sb,Ca,Al,V,Ti,Cd为代表的金属元素群可能是青礞石干预PTZ点燃癫痫模型大鼠的效应物质基础,作用机制可能与这些金属元素群干预影响神经递质的释放及神经元的电平衡、调节离子(Na+,K+,Ca2+等)通道失常诱发的异常同步放电及干预癫痫相关代谢通路等有关,使兴奋与抑制活动相互牵制,最终达到神经元、细胞电平衡等趋于正常生理状态。青礞石组的干预作用总体优于卡马西平组。Objective:To investigate the effect of Chloriti Lapis on metal elements in brain tissue and plasma of epileptic rats kindled by pentylenetetrazol(PTZ),and to explore the possible material basis of Chloriti Lapis. Method: PTZ kindling method was used to establish epileptic rat model. Inductively coupled plasma mass spectrometry(ICP-MS)and inductively coupled plasma optical emission spectrometer(ICP-OES)were used to determine the contents of metal elements in brain tissue and plasma of the blank group,model group,carbamazepine group(0.1 g·kg^(-1))and Chloriti Lapis group(2 g·kg^(-1)). The data were statistically analyzed by SPSS 18.0 software. Result: Compared with the blank group,the contents of Sr,Sb and Ba in brain tissue of rats in the model group were significantly increased(P<0.05,P<0.01),while the contents of Zn,Fe,Cu,K,Li,Co,Sn and Pb were significantly decreased(P<0.05,P<0.01). Compared with the model group,the contents of Zn,Fe,K,Li,Co,As and Pb in brain tissue of rats in the Chloriti Lapis group were obviously increased(P<0.05,P<0.01),while the contents of Sr and Sb were significantly decreased(P<0.01). These results showed that Chloriti Lapis had positive effect on the regulation of the content of metal elements in rat brain tissue to normal level,the intervention effect was clear,and the overall effect was better than that of carbamazepine group. The determination of 21 metal elements in plasma showed that compared with the blank group,the levels of K,Sr and Cd in the model group were significantly increased(P<0.05),and the contents of Li,Al,Ti and Cr were significantly decreased(P<0.05). Compared with the model group,the contents of Ca,K,Li,Al and V in the Chloriti Lapis group were obviously increased(P<0.05,P<0.01),and the contents of Fe,Ti,Sr and Cd were significantly decreased(P<0.05,P<0.01). The correlation analysis of metal elements among the groups showed that there were 17 pairs of elements had positively correlation in the brain tissue of rats,2 pairs of elements had significant negative cor

关 键 词：矿物药 青礞石 癫痫 戊四氮(PTZ) 脑组织 血浆 金属元素 物质基础 

分 类 号：R22[医药卫生—中医基础理论] R969.1[医药卫生—中医学]