靶向抗肿瘤纳米粒的制备及其增强声动力/饥饿联合治疗效果评估  

Preparation of targeted anticancer nanoparticles and evaluation of the efficacy of enhanced synergistic sonodynamic/starvation combined therapy

作　　者：张若 张亮[1] 黄菊 杨扬[1] 王志刚[1] ZHANG Ruo;ZHANG Liang;HUANG Ju;YANG Yang;WANG Zhigang(Department of Ultrasound,the Second Affiliated Hospital,Chongqing Medical University,,Chongqing 400010,China)

机构地区：[1]重庆医科大学附属第二医院超声科,重庆市400010 [2]重庆医科大学附属第三医院超声科

出　　处：《临床超声医学杂志》2021年第5期321-326,共6页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金重点项目(31630026);重庆市技术创新与应用发展专项重点项目(cstc2019jscx-dxwtBX0004)。

摘　　要：目的制备一种载IR780及葡萄糖氧化酶(GOx)的多功能纳米粒,研究其主动靶向能力,并评估其增强体外光声成像及声动力(SDT)/饥饿联合治疗的效果。方法通过双乳化法制备载IR780及GOx的聚乳酸-羟基乙酸共聚物纳米粒(IG@P纳米粒)。检测IG@P纳米粒的理化性质,观察其增强体外光声成像的效果,并评估该纳米粒对鼠源性乳腺癌4T1细胞的靶向能力及体外SDT/饥饿联合治疗效果。结果制备的IG@P纳米粒呈大小均一的球形,平均粒径为(248.1±24.3)nm,且仍保留GOx的催化活性。纳米粒与细胞共孵育后,在鼠源性乳腺癌4T1细胞内可观察到明显的纳米粒聚集,体外治疗结果显示IG@P纳米粒对肿瘤细胞具有显著的杀伤作用,肿瘤细胞存活率为(17.5±9.2)%。结论成功制备了具有肿瘤靶向作用的IG@P多功能纳米粒,其不仅可用于增强光声成像,还可增效SDT/饥饿联合治疗。ObjectiveTo synthesize an IR780 and glucose oxidase(GOx)-based multifunctional nanoparticles,and to examine its abilities of active targeting,photoacoustic(PA)imaging and synergistic sonodynamic(SDT)/starvation therapy.MethodsIR780 and GOx-based PLGA nanoparticles(IG@P NPs)was prepared by double emulsion method.The characteristic properties,targeting ability,PA imaging effect and therapeutic outcome of IG@P NPs were evaluated.ResultsThe average diameters of IG@P NPs with uniform spherical appearance were about(248.1±24.3)nm.And the catalytic activity of GOx in NPs was remained.After co-incubated with 4T1 cells,significant nanoparticles aggregation was observed.In vitro antitumor study showed that IG@P NPs possessed excellent antitumor capability and the survival rate of tumor cells was (17.5±9.2)%.ConclusionMultifunctional IG@P NPs with tumor targeting performance have been successfully prepared,which can be used in PA imaging and SDT/starvation combined therapy.

关 键 词：声动力治疗 饥饿治疗 纳米粒 靶向性 

分 类 号：R730.5[医药卫生—肿瘤]