脊髓半侧液压损伤大鼠动物模型的建立  

Establishment and assessment of fluid-percussion semi-spinal cord injury in rats

作　　者：李景 刘小备 王永利[1] 刘宇恒 于子洋 孙鹏举 赵晓程 权伟[1] 江荣才[1] 张建宁[1] 王增光[1] Li Jing;Liu Xiaobei;Wang Yongli;Liu Yuheng;Yu Ziyang;Sun Pengju;Zhao Xiaocheng;Quan Wei;Jiang Rongcai;Zhang Jianning;Wang Zengguang(Department of Neurosurgery,General Hospital of Tianjin Medical University,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院神经外科,300052

出　　处：《中华实验外科杂志》2021年第5期957-960,共4页Chinese Journal of Experimental Surgery

基　　金：天津市教委科研计划项目(自然科学)(2018ZD03);天津市重点研发计划科技支撑重点项目(20YFZCSY00010)。

摘　　要：目的研究建立一种损伤能量精确、重复性好、损伤分级明确、并发症和死亡率低的脊髓半侧损伤模型。方法选取56只雄性SD大鼠随机进行编号,使用随机数字表将小鼠分为分为轻、中、重3个损伤组(每组n=14)和对照组(n=14)。利用液压打击系统制作轻、中、重各级脊髓损伤模型。各组于建模后3、7、14 d行磁共振检查明确损伤情况,同时于14 d处死取标本行病理检查及免疫组织化学检查。记录实验大鼠操作前后磁刺激诱发的运动诱发电位潜伏期数值,行脊髓损伤运动功能评分(BBB)并进行独立样本t检验。结果根据损伤能量可稳定地制备出轻、中、重型脊髓半侧损伤模型,在损伤后3 d,轻度损伤组大鼠相较于对照组,BBB评分差异有统计学意义[对照组BBB评分为(20.75±0.43)分,轻度损伤组为(18.50±0.93)分,t=26.671,P<0.05],中度损伤组与轻度损伤组比较,差异有统计学意义[中度损伤组(9.34±4.07)分,t=108.824,P<0.05];重度损伤组与中度损伤组比较,差异有统计学意义[重度损伤组(2.50±2.27)分,t=83.290,P<0.05]。磁共振、病理结果可明确显示不同程度的伤后改变。结论脊髓半侧液压损伤动物模型能够精确控制损伤能量,制造分级的脊髓半侧损伤模型。该方法保留部分二便功能,并发症少。Objective To design a stable experimental animal model of fluid-percussion semi-spinal cord injury in rats that could be successfully reproduce and determine different grades of injury degree.Methods A total of 56 SD male rats were randomly numbered,and the rats were divided into four groups:mild,middle and severe injury group and control group(n=14 for each group)by using a random number table.Standard operational procedures were performed on rats.The model was used to evaluate neurological function score and motor potential evoked by magnetic stimulation at 3,7 and 14 d after injury.A T-test was done to determine if there was a statistically significant difference.Pathological and immunohistochemical analyses were done 14 days after injury.Results Neurological function scores in the mild injury group were significantly different from those in the control group at the same time[for Basso,Beattie&Bresnahan locomotor rating(BBB)scale:20.75±0.43 in the control group,and 18.50±0.93 in the mild injury group,t=26.671,P<0.05],and those in the middle injury group were significantly different from those in the mild injury group at the same time(9.34±4.07 in the middle injury group,t=108.824,P<0.05).There were statistically significant differences between the severe injury group and the moderate injury group at the same time(2.50±2.27 in the severe injury group,t=83.290,P<0.05).The magnetic resonance imaging and pathological check revealed that the area of injury was enlarged with an increase in the injured energy.Conclusion This model has a low ratio of intercurrent syndromes and a high long-life ratio.

关 键 词：脊髓损伤 模型 动物 运动诱发电位 核磁共振成像 

分 类 号：R-332[医药卫生] R651.2