以4-香豆酸为底物转化白藜芦醇大肠杆菌工程菌的构建  

Construction of engineered Escherichia coli for resveratrol synthesis using 4-coumaric acid as substrate

作　　者：翟逸 李明 赵秀敏 张晓利 南化征 蒋龙声 江旭 阮志勇[3] ZHAI Yi;LI Ming;ZHAO Xiumin;ZHANG Xiaoli;NAN Huazheng;JIANG Longsheng;JIANG Xu;RUAN Zhiyong(State and Local Joint Engineering Laboratory of Recombinant Protein and Gene Detection Technology,Shandong Boaoke Biotechnology Co.,LTD,Liaocheng 252000,Shandong,China;Yibin Shan Gougou Agricultural Science and Technology Co.Ltd,Yibin 644000,Sichuan,China;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]山东博奥克生物科技有限公司重组蛋白基因检测技术国家地方联合工程实验室,山东聊城252000 [2]宜宾山勾勾农业科技有限公司,四川宜宾644000 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《生物资源》2021年第2期194-199,共6页Biotic Resources

基　　金：山东博奥克生物科技有限公司博士后项目(bsh151210);宜宾市科技计划项目(2019YG01)。

摘　　要：白藜芦醇具有多种生物活性,在食品、化妆品及生物制药中具有广阔的应用前景。利用合成生物学生产白藜芦醇是未来的发展趋势。将白藜芦醇合酶(resveratrol synthase,RS)和4-香豆酰-CoA连接酶(4-coumaric acid-CoA ligase,4CL)的编码基因整合到共表达载体pETDuet-1上成功构建了重组质粒pETDuet-rs-4cl,转化大肠杆菌BL21(DE3),在M9培养基中添加4-香豆酸,在诱导条件下培养,发酵液中白藜芦醇含量为8.6 mg/L。本研究在大肠杆菌中实现了白藜芦醇的转化,为微生物法生产白藜芦醇奠定了基础。Resveratrol has multiple biological activities and broad application prospects in food,cosmetics and biopharmaceutical industries.The production of resveratrol using synthetic biology is the trend of development.Resveratrol synthase encoding gene rs and 4-coumaric acid-CoA ligase encoding gene 4cl were inserted into co-expression vector pET-Duet-1 and recombinant vector pETDuet-rs-4 cl was successfully constructed.The recombinant vector was transformed into Escherichia coli BL21(DE3).The 8.6 mg/L of resveratrol was determined in M9 fermentation broth with additional 4-coumaric acid and inducer.In this study,resveratrol is synthesized in E.coli,which lays a foundation for the production of resveratrol by microbial method.

关 键 词：白藜芦醇 大肠杆菌 重组工程菌 微生物发酵 

分 类 号：Q789[生物学—分子生物学]