微小RNA-938在肝细胞癌中的表达及对肝癌细胞增殖的影响  

Expression of microRNA-938 and its effect on cell proliferation in hepatocellular carcinoma

作　　者：陈杰[1] 朱冬梅 徐阳 朱斌[1] 邵伟斌[1] Chen Jie;Zhu Dongmei;Xu Yang;Zhu Bin;Shao Weibin(Department of General Surgery,Second Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China)

机构地区：[1]南通大学第二附属医院普外科,南通226001

出　　处：《中华肝胆外科杂志》2021年第5期335-338,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：南通市卫计委青年科研基金(WKZL2018014,QA2019004)。

摘　　要：目的探讨微小RNA-938(miR-938)在肝癌组织中的表达情况及其对肝癌细胞增殖的影响和调控机制。方法收集2015年1月至2019年6月在南通大学第二附属医院就诊并手术切除的40例肝细胞癌患者肝癌组织和癌旁组织,其中男性25例,女性15例,平均年龄61.4岁。miR-938过表达组HepG2肝癌细胞转染miR-938模拟物,阴性对照组转染阴性对照序列。采用试剂盒检测细胞增殖情况,荧光定量聚合酶链反应检测miR-938及琥珀酸脱氢酶复合体亚基D(SDHD)的表达,Western印迹检测SDHD蛋白表达。双荧光素酶报告实验验证miR-938的靶基因。结果肝癌组织中miR-938相对表达量为(0.060±0.002),高于癌旁组织(0.030±0.002),差异有统计学意义(P<0.05)。肝癌组织中SDHD mRNA相对表达量为(0.028±0.002),低于癌旁组织(0.062±0.002),SDHD蛋白相对表达为(0.963±0.008),低于癌旁组织(1.083±0.037),差异均有统计学意义(均P<0.05)。miR-938过表达组细胞增殖活力明显高于阴性对照组,差异有统计学意义(P<0.05)。双荧光素酶报告结果显示只有miR-938模拟物和pGL3-SDHD野生型质粒组荧光素酶活性下降。阴性对照组SDHD mRNA和蛋白相对表达量均高于过表达组,差异有统计学意义(P<0.05)。结论miR-938在肝细胞癌患者肝癌组织中高表达,miR-938可能通过抑制SDHD的表达,促进肝癌细胞增殖。Objective To investigate the expression of microRNA(miR)-938,its effect on cell proliferation and its regulatory mechanism in hepatocellular carcinoma(HCC).Methods HCC and paracancerous tissues were collected from 40 patients with HCC who underwent surgical resection in the Second Affiliated Hospital of Nantong University from January 2015 to June 2019,including 25 males and 15 females,with an average age of 61.4 years.HepG2 cells in the miR-938 overexpression group were transfected with miR-938 mimics,and the negative control group was transfected with the negative control sequence.Cell proliferation was detected by kit,the expression of miR-938 and the succinate dehydrogenase complex subunit D(SDHD)was detected by fluorescence quantitative polymerase chain reaction,and SDHD protein expression was detected by Western blot.The target genes of miR-938 were verified by dual luciferase reporting.Results The relative expression of miR-938 in HCC tissues was(0.060±0.002),which was higher than that in adjacent tissues(0.030±0.002),and the difference was statistically significant(P<0.05).The mRNA relative expression of SDHD in HCC tissues was(0.028±0.002),lower than that in adjacent tissues(0.062±0.002),and the protein expression of SDHD in HCC tissues was(0.963±0.008),lower than that in adjacent tissues(1.083±0.037),with statistical significance(both P<0.05).The proliferation activity of miR-938 overexpression group was significantly higher than that of negative control group,and the difference was statistically significant(P<0.05).MiR-938 significantly inhibited the luciferase activity of SDHD wild-type 3’-untranslated regions.In the overexpression miR-938 cells,SDHD mRNA and protein levels were significantly lower than those in the negative control group(P<0.05).Conclusion MiR-938 was highly expressed in HCC tissues.MiR-938 promoted the proliferation of HCC cells by inhibiting the expression of SDHD.

关 键 词：微RNAS 癌 肝细胞 细胞增殖 琥珀酸脱氢酶复合体亚基D 

分 类 号：R735.7[医药卫生—肿瘤]