胺脱氢酶在手性胺生物合成中的研究进展  

Research Progress on the Biosynthesis of Chiral Amine Compounds Using Amine Dehydrogenase

作　　者：郑晨妮 吴刚 贺瑾 陈蕊蕊 王志国[3] 陈振明 ZHENG Chenni;WU Gang;HE Jin;CHEN Ruirui;WANG Zhiguo;CHEN Zhenming(College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China;Zhejiang Haisen Pharmaceutical Co.Ltd,Dongyang 322100,China;Division of Health Sciences,Hangzhou Normal University,Hangzhou 311121,China)

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州311121 [2]浙江海森药业股份有限公司,浙江东阳322100 [3]杭州师范大学医学部,浙江杭州311121

出　　处：《杭州师范大学学报（自然科学版）》2021年第3期230-238,共9页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省科技厅科研项目(2017C1128);杭州市科技局科研项目(20170533B03).

摘　　要：手性胺化合物广泛存在于自然界各种天然产物中,具有易形成氢键和功能多样性的特点,是多种药物活性物质和农药的合成前体.酶法是手性胺的主要合成方法之一,近年来以其高产率、高手性选择性和环境友好等优势成为手性胺研究领域关注的焦点.文章系统总结了胺脱氢酶在手性胺合成反应中的循环方式,从反应机制和动力学角度阐述了不同类型胺脱氢酶催化合成手性胺的反应特性,并展望了其在工业生产领域的应用前景.Chiral amine compounds, which are widely present in various natural products, are the synthetic precursors of a variety of active substance and pesticides because of its characteristics of easy formation of hydrogen bonds and functional diversity. Enzymatic method is one of the main synthesis methods for chiral amines. In recent years, it has become the focus of chiral amines researches because of its high yield, high chiral selectivity and environmental friendliness. This review systematically summarizes the circulation of amine dehydrogenase in chiral amine synthesis reactions, explains the reaction characteristics of different types of amine dehydrogenase from the perspectives of catalytic mechanism and reaction kinetics, and prospects its application future in the industrial production.

关 键 词：手性胺 胺脱氢酶 手性选择性 生物合成 

分 类 号：O555.6[理学—热学与物质分子运动论]