睡眠剥夺后大鼠下丘脑Orexin-A神经元的表达及其对学习记忆的影响  

The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory

作　　者：倪丽艳 唐吉友 Ni Liyan;Tang Jiyou(Juye County People's Hospital,Juye 274900,China;The First Affiliated Hospital of Shandong First Medical University,Jinan 250014,China)

机构地区：[1]巨野县人民医院,山东巨野274900 [2]山东第一医科大学第一附属医院,山东济南250014

出　　处：《山东第一医科大学（山东省医学科学院）学报》2021年第5期380-384,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：国家自然科学基金(81471345)。

摘　　要：目的探讨大鼠睡眠剥夺后下丘脑Orexin-A的表达及其对空间学习记忆能力的影响。方法健康成年雄性Wistar大鼠随机分为空白对照组(CC)、环境对照组(TC)、睡眠剥夺组(SD)。采用间歇性跑步机进行72 h全睡眠剥夺。免疫组化观察各组大鼠下丘脑Orexin-A表达,Morris水迷宫试验测试各组大鼠的空间学习记忆能力。结果SD组大鼠下丘脑Orexin-A阳性细胞数较TC和CC组显著增加,差异有统计学意义(P<0.01),TC组与CC组相比差异无统计学意义(P>0.05);与TC组相比,SD组逃避潜伏期明显延长,穿越平台象限的次数明显减少,差异有统计学意义(P<0.01),TC与CC组比较差异无统计学意义(P>0.05)。结论睡眠剥夺可能通过诱导下丘脑Orexin-A的过度表达损害大鼠的空间学习记忆能力。Objective:To investigate the expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory.Methods:Healthy adult male Wistar rats were randomly divided into cage control(CC),treadmill control(TC),sleep deprivation(SD)group.72h total sleep deprivation was performed by intermittent treadmill.The expression of Orexin-A was observed by immunohistochemistry,and the spatial learning and memory ability was tested by Morris water maze test.Results:Compared with TC,the number of Orexin-A positive cells in hypothalamus of SD rats was significantly increased(P<0.01).There was no significant difference between TC and CC group(P>0.05).Compared with TC group,the escape latency and the times of crossing platform quadrant in SD group were significantly longer(P<0.01),and there was no significant difference between TC group and CC group(P>0.05).Conclusion:sleep deprivation may impair spatial learning and memory in rats by inducing overexpression of Orexin-A in hypothalamus.

关 键 词：睡眠剥夺 学习记忆 OREXIN-A 

分 类 号：R741.02[医药卫生—神经病学与精神病学]