家族聚集性与散发性LAA脑梗死患者CYP2C19及GPⅡb/Ⅲa基因表达差异研究  

Study on the difference in expression of CYP2C19 and GPⅡb/Ⅲa between patients with familial LAA atherosclerotic cerebral infarction and those with sporadic LAA cerebral infarction

作　　者：郝瑞 许双双 高敏 王燕华 吕玉芹 王娜 张敬军 Hao Rui;Xu Shuangshuang;Gao Min;Wang Yanhua;Lv Yuqin;Wang Na;Zhang Jingjun(Taishan Hospital of Shandong Province,Taian 271000,China;The Second Affiliated Hospital of Shandong First Medical University,Taian 271000,China)

机构地区：[1]山东省泰山医院,山东泰安271000 [2]山东第一医科大学第二附属医院,山东泰安271000

出　　处：《山东第一医科大学（山东省医学科学院）学报》2021年第5期385-389,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：山东省高等学校科技计划(J15LL07);山东第一医科大学学术提升计划(2019QL013);泰安市科技局计划(2017NS0248)。

摘　　要：目的探讨家族聚集性大动脉粥样硬化性(large-artery atherosclerosis,LAA)脑梗死患者与散发性LAA脑梗死患者ADP、CYP2C19、P2Y12受体及GPⅡb/Ⅲa等表达差异。方法收集家族聚集性LAA脑梗死和散发性LAA脑梗死患者各25例分别为家族组及散发组,选择25例健康人群作为对照组。分别检测ADP蛋白表达量、CYP2C19基因、P2Y12基因G52T位点及GPⅡb/Ⅲa蛋白表达量。结果家族组与对照组比较ADP表达量差异具有统计学意义(P<0.05),家族组与散发组及散发组与对照组比较ADP表达量差异无统计学意义(P>0.05);家族组与对照组比较CYP2C19基因表达明显下调,差异具有统计学意义(P<0.05);散发组与对照组比较CYP2C19基因表达差异无统计学意义(P>0.05);P2Y12基因的G52T位点在家族组、散发组和对照组之间差异有统计学意义(P<0.05);家族组与对照组比较GPⅡb/Ⅲa表达量明显上调,差异有统计学意义,家族组与散发组及散发组与对照组比较GPⅡb/Ⅲa表达量差异无统计学意义(P>0.05);家族组及散发组治疗3个月后mRS评分和神经功能缺损恢复情况比较差异均有统计学意义(P<0.05)。结论家族组患者与散发组患者ADP蛋白表达、CYP2C19基因、P2Y12基因G52T位点及GPⅡb/Ⅲa蛋白表达等存在明显差异。Objective:To investigate the differences in expression of ADP,CYP2C19,P2Y12 receptors and GPⅡb/Ⅲa between patients with familial atherosclerotic(large-artery atherosclerosis,LAA)cerebral infarction and those with sporadic LAA cerebral infarction.Methods:Twenty-five patients with familial aggregated LAA cerebral infarction and sporadic LAA cerebral infarction were collected as family group and sporadic group respectively.Twenty-five healthy people were selected as control group.ADP protein expression,CYP2C19 gene,P2Y12 gene G52T locus and GPⅡb/Ⅲa protein expression were detected respectively.Results:There was significant difference in ADP expression between the family group and the control group;There was no significant difference between the family group and the sporadic group and between the sporadic group and the control group;Compared with the control group,the expression of CYP2C19 genes in the family group was significantly down-regulated,and the difference was statistically significant;Compared with the control group,the expression of CYP2C19 genes in the sporadic group had no significant difference in gene expression;The G52T loci of P2Y12 gene were significantly different between family group,sporadic group and control group.The expression of GPⅡb/Ⅲa in family group and control group was significantly up-regulated,and the difference was statistically significant.The difference in the expression of GPⅡb/Ⅲa.between family group and sporadic group and between sporadic group and control group was not significant;After 3 months of treatment,mRS scores of the family group and the sporadic group were compared,and the difference between the two groups was statistically significant.Conclusion:There are significant differences in ADP protein expression,CYP2C19 gene,P2Y12 gene G52T locus and GPⅡb/Ⅲa protein expression between family group and sporadic group.

关 键 词：脑血管病 大动脉粥样硬化性脑梗死 基因 CYP2C19 

分 类 号：R739.41[医药卫生—肿瘤]