顺铂腹腔热灌注化疗对人胃癌细胞系凋亡的影响及其作用机制  

Effect of cisplatin intraperitoneal hyperthermic perfusion chemotherapyon apoptosis of human gastric cancer cell lines and its mechanism

作　　者：王法宝 夏泽亮[1] 褚亮[1] 周少波[1] 许兆龙 蒋磊[1] 单二波[1] 周超毅 Wang Fabao;Xia Zeliang;Chu Liang;Zhou Shaobo;Xu Zhaolong;Jiang Lei;Shan Erbo;Zhou Chaoyi(The Second Affiliated Hospital of Bengbu Medical College,Bengbu 233000,China)

机构地区：[1]蚌埠医学院第二附属医院,安徽蚌埠233000

出　　处：《山东第一医科大学（山东省医学科学院）学报》2021年第5期396-400,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：蚌埠医学院自然科学项目(BYKY2019153ZD,BYKY2019136ZD,BYKY2019138ZD)。

摘　　要：目的初步探究顺铂腹腔热灌注对人胃癌细胞系(SGC-7901)凋亡的影响及其作用机制。方法实验分为对照组、顺铂组、热疗组和热化疗组。CCK-8检测各组细胞活力,Hoechst染色法检测细胞凋亡,流式细胞仪检测细胞凋亡率,Western blot检测Caspase-3、Bcl-2、Bax和Bid蛋白表达水平。结果与对照组比较,顺铂组、热疗组和热化疗组细胞活力显著降低(P<0.01),细胞凋亡率显著升高(P<0.01),Caspase-3、Bax和Bid蛋白显著升高(P<0.05或P<0.01),Bcl-2表达下调(P<0.01)。与顺铂组和热疗组相比,热化疗组细胞活力明显降低(P<0.05),细胞凋亡率显著升高(P<0.05),Caspase-3、Bax和Bid蛋白显著升高(P<0.05),Bcl-2表达显著下调(P<0.05)。顺铂组与热疗组差异无统计学意义(P>0.05)。结论顺铂腹腔热灌注化疗能够通过促进Caspase-3、Bax和Bid表达,下调Bcl-2,促进SGC-7901细胞凋亡。Objective:To preliminarily explore the effect of intraperitoneal hyperthermic perfusion of cisplatin on the apoptosis of human gastric cancer cell line(SGC-7901)and its mechanism.Methods:The four groups divided in the experiment were the control group,cisplatin group,hyperthermia group and hyperthermia chemotherapy group.CCK-8 was used to detect cell viability in each group,Hoechst staining method was used to detect cell apoptosis,flow cytometry was used to detect cell apoptosis rate,and Western blot was used to detect the expression levels of Caspase-3,Bcl-2,Bax and Bid proteins.Results:Compared with the control group,the cell viability of the cisplatin group,the hyperthermia group and the thermochemotherapy group was significantly reduced(P<0.01),the apoptosis rate was significantly increased(P<0.01),and the Caspase-3,Bax and Bid proteins were significantly increased High(P<0.05 or P<0.01),Bcl-2 expression was down-regulated(P<0.01).Compared with the cisplatin group and the hyperthermia group,the cell viability of the thermochemotherapy group was significantly reduced(P<0.05),the apoptosis rate was significantly increased(P<0.05),and the Caspase-3,Bax and Bid proteins were significantly increased(P<0.05),the expression of Bcl-2 was significantly down-regulated(P<0.05).There was no significant difference between the cisplatin group and the hyperthermia group(P>0.05).Conclusion:Cisplatin intraperitoneal hyperthermic perfusion chemotherapy can promote the apoptosis of SGC-7901 cells by promoting the expression of Caspase-3,Bax and Bid and down-regulating Bcl-2.

关 键 词：胃癌 顺铂腹腔热灌注 细胞凋亡 

分 类 号：R73-36[医药卫生—肿瘤]