Palm oil protectsα-linolenic acid from rumen biohydrogenation and muscle oxidation in cashmere goat kids  

作　　者：Xue Wang Graeme B.Martin Qi Wen Shulin Liu Yinhao Li Binlin Shi Xiaoyu Guo Yanli Zhao Yangdong Guo Sumei Yan 

机构地区：[1]Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China [2]Beijing Advanced Innovation Center for Food Nutrition and Human Health,College of Horticulture,China Agricultural University,Beijing 100193,China [3]UWA Institute of Agriculture,The University of Western Australia,35 Stirling Highway,Crawley,WA 6009,Australia

出　　处：《Journal of Animal Science and Biotechnology》2021年第2期711-728,共18页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Science Foundation of China(Project No.31760685);the National Key R&D Program of China(Project No.2017YFD0500504).

摘　　要：Background:In ruminants,dietary C18:3n-3 can be lost through biohydrogenation in the rumen;and C18:3n-3 that by-passes the rumen still can be lost through oxidation in muscle,theoretically reducing the deposition of C18:3n-3,the substrate for synthesis of poly-unsaturated fatty acids(n-3 LCPUFA)in muscle.In vitro studies have shown that rumen hydrogenation of C18:3n-3 is reduced by supplementation with palm oil(rich in cis-9 C18:1).In addition,in hepatocytes,studies with neonatal rats have shown that cis-9 C18:1 inhibits the oxidation of C18:3n-3.It therefore seems likely that palm oil could reduce both rumen biohydrogenation of C18:3n-3 and muscle oxidation of C18:3n-3.The present experiment tested whether the addition of palm oil to a linseed oil supplement for goat kids would prevent the losses of C18:3n-3 and thus improve the FA composition in two muscles,Longissimus dorsi and Biceps femoris.To investigate the processes involved,we studied the rumen bacterial communities and measured the mRNA expression of genes related to lipid metabolism in Longissimus dorsi.Sixty 4-month-old castrated male Albas white cashmere kids were randomly allocated among three dietary treatments.All three diets contained the same ingredients in the same proportions,but differed in their fat additives:palm oil(PMO),linseed oil(LSO)or mixed oil(MIX;2 parts linseed oil plus 1 part palm oil on a weight basis).Results:Compared with the LSO diet,the MIX diet decreased the relative abuandance of Pseudobutyrivibrio,a bacterial species that is positively related to the proportional loss rate of dietary C18:3n-3 and that has been reported to generate the ATP required for biohydrogenation(reflecting a decrease in the abundance of rumen bacteria that hydrogenate C18:3n-3 in MIX kids).In muscle,the MIX diet increased concentrations of C18:3n-3,C20:5n-3,C22:6n-3,and n-3 LCPUFA,and thus decreased the n-6/n-3 ratio;decreased the mRNA expression of CPT1β(a gene associated with fatty acid oxidation)and increased the mRNA expression of FADS1 and FADS

关 键 词：DESATURASES Docosahexaenoic acid Hydrogenation Goats MEAT Oleic acid Oxidative stress 

分 类 号：S827.5[农业科学—畜牧学]