核苷酸结合寡聚化结构域样受体蛋白3炎症小体与阿尔茨海默病患者神经炎症反应、氧化应激的相关性分析  

Correlation of nucleotide-bindingoligomerization domain-like receptor 3 inflamma⁃some with neuroinflammatory response and oxidative stress in patients with Alzheim⁃er’s disease

作　　者：师强 郑莹莹[1] 杨增烨[1] SHI Qiang;ZHENG Ying-Ying;YANG Zeng-Ye Yan’an(University Affiliated Hospital,Yan’an,Shanxi 716000,China)

机构地区：[1]延安大学附属医院,陕西延安716000

出　　处：《国际神经病学神经外科学杂志》2021年第2期115-119,共5页Journal of International Neurology and Neurosurgery

基　　金：陕西省教育厅专项科研计划项目(19JK0972)。

摘　　要：目的探究核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)炎症小体信号通路相关因子表达与阿尔茨海默病(AD)患者神经炎症反应及损伤和氧化应激的相关性。方法选取该院2017年5月—2019年5月收治的116例AD患者,依照简易精神状态量表(MMSE)评分为轻度组(53例)、中度组(45例)和重度组(18例),另选取同期于该院进行健康体检的40例健康体检者作为对照组,对比各组日常生活能力量表(ADL)、阿尔茨海默病评定量表-认知(ADAS-Cog)评分。检测比较NLRP3炎症小体信号通路相关因子[NLRP3、白细胞介素(IL)-1β及胱天蛋白酶(caspase)-1)]mRNA表达,炎症反应及损伤相关因子[核因子-κB(NF-κB)、神经胶质纤维酸性蛋白(GFAP)及IL-18]及氧化应激相关因子[超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GPX-3)]水平,分析NLRP3炎症小体信号通路相关因子与炎症反应及损伤和氧化应激的相关性。结果不同严重程度AD患者ADL评分及ADAS-Cog评分比较,差异具有统计学意义(P<0.05);且随着AD病情加重,患者ADL评分逐渐减低,ADAS-Cog评分升高(P<0.05)。AD患者NLRP3、IL-1β及Caspase-1 mRNA表达水平高于对照组(P<0.05),且随着病情加重,NLRP3、IL-1β及Cas⁃pase-1 mRNA表达水平逐渐升高(P<0.05)。AD患者炎症反应及损伤因子NF-κB、GFAP及IL-18水平高于对照组(P<0.05),且随着病情加重,NF-κB、GFAP及IL-18水平逐渐升高(P<0.05)。AD患者氧化应激因子SOD、CAT及GPX-3低于对照组(P<0.05),MDA高于对照组,且不同严重程度AD患者各指标比较,差异具有统计学意义(P<0.05)。相关性分析结果显示,NLRP3、IL-1β及Caspase-1 mRNA表达水平与炎症反应及损伤因子NF-κB、GFAP及IL-18表达呈正相关(P<0.05),与氧化应激相关因子SOD、CAT及GPX-3呈负相关,与MDA表达呈水平呈正相关(P<0.05)。NLRP3、IL-1β及Caspase-1 mRNA表达水平与AD患者ADL评分呈负相关,与ADAS-Cog评分呈正相关Objective To investigate the correlation of the expression of nucleotide-binding oligomerization domain-like receptor 3(NLRP3) inflammasome signaling pathway-associated factors with neuroinflammatory response,neuroinflammatory injury,and oxidative stress in patients with Alzheimer’s disease(AD).Methods A total of 116 patients with AD who were admitted to our hospital from May 2017 to May 2019 were enrolled,and according to the Mini-Mental State Examination(MMSE) score,the patients were divided into mild group with 53 patients,moderate group with 45 patients,and severe group with 18 patients;40 individuals who underwent physical examination in our hospital during the same period of time were enrolled as control group.The above groups were compared in terms of Activities of Daily Living(ADL) score and Alzheimer’s Disease Assessment Scale-cognitive subscale(ADAS-Cog) score.The correlation of NLRP3 inflammasome signaling pathway-associated factors with inflammatory response,inflammatory injury,and oxidative stress was analyzed by measuring and comparing the mRNA expression of NLRP3 inflammasome signaling pathway-associated factors [NLRP3,interleukin-1β(IL-1β),and caspase-1],the levels of factors associated with inflammatory response and injury [nuclear factorkappa B(NF-κB),glial fibrillary acidic protein(GFAP),and interleukin-18(IL-18)],and the levels of oxidative stress-related factors [superoxide dismutase(SOD),malondialdehyde(MDA),catalase(CAT),and glutathione peroxidase(GPX-3)].Results There were significant differences in ADL and ADAS-Cog scores between the patients with different severities of AD(P<0.05),and ADL score gradually decreased and ADAS-Cog score gradually increased with the aggravation of AD(P<0.05).The patients with AD had significantly higher mRNA expression levels of NLRP3,IL-1β,and caspase-1 than the control group(P<0.05),and the mRNA expression levels of NLRP3,IL-1β,and caspase-1 gradually increased with the aggravation of the disease(P<0.05).The patients with AD had significantly highe

关 键 词：阿尔茨海默病 核苷酸结合寡聚化结构域样受体蛋白3 炎症小体 神经炎症 氧化应激 

分 类 号：R741[医药卫生—神经病学与精神病学]