清胰通腑泻热饮对重症急性胰腺炎患者外周血单核细胞p38MAPK/NF-κB信号通路及下游炎症因子的影响  

Effect of Qingyi Tongfu Xiere Yin on p38MAPK/NF-κB Signaling Pathway and Downstream Inflammatory Factors in Peripheral Blood Monocytes of Patients with Severe Acute Pancreatitis

作　　者：邵兴[1] 向晶[1] 童洪杰[1] 邵利梅 陈琨[1] SHAO Xing;XIANG Jing;TONG Hongjie;SHAO Limei;CHEN Kun

机构地区：[1]金华市中心医院重症医学科,浙江金华321000

出　　处：《新中医》2021年第8期59-62,共4页New Chinese Medicine

基　　金：金华市中医药科学技术研究计划项目(2019jzk015)。

摘　　要：目的:观察清胰通腑泻热饮对重症急性胰腺炎(SAP)患者外周血单核细胞p38MAPK/核因子-κB(NF-κB)信号通路及下游炎症因子的影响并探讨其作用机制。方法:将60例患者随机分为对照组与观察组各30例。对照组给予西医常规治疗,观察组在对照组的基础上加用清胰通腑泻热饮,疗程均为10 d。2组分别在治疗第1天、第11天早晨7点抽血,采用Western blot法测定外周血单核细胞p38MAPK和磷酸化p38MAPK(p-p38MAPK)蛋白表达水平;酶联免疫吸附法(ELISA)测定肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、NF-κB水平。结果:观察组总有效率为93.33%,高于对照组的70.00%(P<0.05)。与同组治疗前比较,治疗后2组p38MAPK、p-p38MAPK蛋白表达及TNF-α、IL-6、IL-8、NF-κB水平均下降(P<0.05),且观察组低于对照组(P<0.05)。结论:SAP患者外周血单核细胞p38MAPK、p-p38MAPK蛋白表达水平及TNF-α、IL-6、IL-8、NF-κB水平均升高;清胰通腑泻热饮可能通过下调p38MAPK/NF-κB水平抑制下游炎症因子的释放。Objective:To observe the effect of Qingyi Tongfu Xiere yin on p38MAPK/nuclear factor-κB(NF-κB)signaling pathway and downstream inflammatory factors in peripheral blood monocytes of patients with severe acute pancreatitis(SAP)and to explore its mechanism.Methods:A total of 60 cases of patients were randomly divided into the control group and the observation group,30 cases in each group.The control group was given routine western medicine treatment,and the observation group was additionally given Qingyi Tongfu Xiere yin based on the treatment of the control group.Both groups were treated for 10 days.At 7:00 am on the 1st and 11th days of treatment respectively in the two groups,blood samples were collected to measure the protein expression levels of p38MAPK and phosphorylated p38MAPK(p-p38MAPK)in peripheral blood monocytes by Western blot.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8)and NF-κB were measured by enzyme linked immunosorbent assay(ELISA).Results:The total effective rate was 93.33%in the observation group,higher than that of 70.00%in the control group(P<0.05).After treatment,the levels of protein expressions of p38MAPK and p-p38MAPK as well as TNF-α,IL-6,IL-8 and NF-κB in peripheral blood monocytes in the two groups were decreased when compared with those before treatment(P<0.05),and the levels in the observation group were lower than those in the control group(P<0.05).Conclusions:The levels of protein expressions of p38MAPK and p-p38MAPK as well as TNF-α,IL-6,IL-8 and NF-κB in peripheral blood monocytes of SAP patients are increased.Qingyi Tongfu Xiere yin may inhibit the release of downstream inflammatory factors by down-regulating the level of p38MAPK/NF-κB.

关 键 词：重症急性胰腺炎 清胰通腑泻热饮 P38MAPK信号通路 核因子-κB 炎症因子 

分 类 号：R576[医药卫生—消化系统]