马铃薯StSN2的克隆、定位及表达分析  

Cloning,Location and Expression Analysis of Gene StSN2 in Solanum tuberosum

作　　者：彭洁[1] 邓孟胜 张杰[1] 刘石锋 罗李飞 王西瑶[1] PENG Jie;DENG Meng-sheng;ZHANG Jie;LIU Shi-feng;LUO Li-fei;WANG Xi-yao(College of Agronomy,Sichuan Agricultural University,Chengdu 611130;Leshan Seed Control Station,Leshan 614000)

机构地区：[1]四川农业大学,成都611130 [2]乐山市种子管理站,乐山614000

出　　处：《生物技术通报》2021年第5期11-18,共8页Biotechnology Bulletin

基　　金：国家现代农业产业技术体系四川薯类创新团队项目(四川省农业厅[2019]59号)。

摘　　要：Snakin-2(StSN2)属于Snakin/GASA家族,GASAs在调控植物生长发育与抗逆性具有重要作用。克隆StSN2,研究StSN2在马铃薯块茎休眠过程中的功能,为进一步探究StSN2与马铃薯块茎休眠的关系提供理论依据。以马铃薯品种"川芋10号"为材料,克隆StSN2全长CDS序列,并进行生物信息学分析,再利用qRT-PCR分析其组织表达特异性、休眠释放过程表达变化以及对激素的响应情况。结果表明,StSN2开放阅读框为315 bp,编码104个氨基酸,相对分子质量约为11.04 kD,理论等电点为8.91;马铃薯StSN2与番茄、茄子、烟草等茄科植物亲缘关系较近;烟草瞬时表达结果显示该蛋白定位于细胞核、质膜;组织表达分析表明,StSN2在各组织中均有表达,以块茎最高,其次是叶片、根,在茎、花蕾、叶柄最低;进一步研究发现,StSN2表达受ABA、BR以及GA_3调控,尤其是显著响应休眠激素ABA的诱导,并且其表达随休眠的释放而降低,表明StSN2可能参与激素对马铃薯休眠的调控。Snakin-2(StSN2)belongs to the Snakin/GASA family.GASAs play an important role in regulating plant growth and stress resistance.This work aims to clone the StSN2 gene,to study the function of StSN2 in the process of Solanum tuberosum tuber dormancy,and to explore the relationship between StSN2 and S.tuberosum tuber dormancy.The full-length CDS sequence of StSN2 was cloned from potato variety"Chuanyu 10"and analyzed by bioinformatics.The tissue expression specificity,dormancy release process and response to hormone were analyzed by qRT-PCR.The results showed that the open reading frame of the gene was 315 bp,encoding 104 amino acids,the relative molecular mass was about 11.04 kD,and the theoretical isoelectric point was 8.91;the protein was closely related to tomato,eggplant,tobacco and other Solanaceae plants in evolution.The results of tobacco transient expression revealed that the protein was localized in the nucleus and plasma membrane.Tissue expression analysis demonstrated that StSN2 was expressed in all tissues,with tubers being the highest,followed by leaves and roots,and lowest in stems,flower buds,and petioles.Further research discovered that the expression of StSN2 was regulated by ABA,BR and GA3,especially in response to the induction of the dormant hormone ABA,and its expression decreased with the release of dormancy.In conclusion,StSN2 may be involved in hormone regulation of S.tuberosum dormancy.

关 键 词：马铃薯 StSN2 块茎休眠 基因克隆 表达分析 激素 

分 类 号：S532[农业科学—作物学] Q943.2[生物学—植物学]