活化因子TEPP-46对胃癌细胞MGC-803侵袭、增殖的影响及机制  

Effect and mechanism of TEPP-46 on invasion and proliferation of gastric cancer cell MGC-803

作　　者：朱代亮 许建利 ZHU Dai-liang;XU Jian-li(Department of General Surgery,Jiangjin Central Hospital of Chongqing,Chongqing 402260,China)

机构地区：[1]重庆市江津区中心医院普外科,重庆402260

出　　处：《中国现代普通外科进展》2021年第5期337-341,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨活化因子TEPP-46对胃癌细胞MGC-803侵袭、增殖的影响及分子机制。方法:Western blot和细胞免疫荧光实验检测胃癌细胞株MGC-803及正常胃粘膜细胞GES-1中PKM2的表达;用TEPP-46预处理MGC-803细胞后,Transwell侵袭实验检测细胞侵袭能力,将MGC-803细胞种植于BALB/c裸鼠皮下,10 d后取下皮下瘤,测量皮下瘤大小,免疫组化检测增殖细胞核抗原PCNA在皮下瘤中的表达;用TEPP-46预处理MGC-803细胞后,Western blot检测细胞中PKM2、HIF-1α、p-NF-κB及NF-κB的蛋白表达。结果:GES-1细胞PKM2四聚体高表达,MGC-803细胞中PKM2以单聚体表达为主,四聚体表达显著降低;预处理TEPP-46抑制MGC-803细胞的侵袭和增殖能力;预处理TEPP-46促进PKM2四聚体表达,抑制HIF-1α和p-NF-κB的表达。结论:活化因子TEPP-46通过抑制PKM2核转位,促进PKM2四聚体表达,抑制HIF-1α和p-NF-κB的表达,进而抑制胃癌细胞的侵袭和增殖。Objective:To investigate the effect of activating factor TEPP-46 on invasion and proliferation of gastric cancer cell MGC-803 and its molecular mechanism.Methods:Western blot and cellular immunofluorescence assay were used to detect the expression of PKM2 in gastric cancer cell line MGC-803 and normal gastric mucosa cell GES-1;After pretreatment of MGC-803 cells with TEPP-46,Transwell invasion test was used to detect the invasion ability of cells.MGC-803 cells were planted under the skin of nude mice.Subcutaneous tumors were removed 10 days later,the quality of subcutaneous tumors was measured,and the expression of proliferating cell nuclear antigen PCNA in subcutaneous tumors was detected by immunohistochemistry;Western blot was used to detect the protein expression of PKM2,HIF-1α,p-NF-κB and NF-κB in cells.Results:The expression of PKM2 tetramer in GES-1 cells is high,and the expression of PKM2 in MGC-803 cells is mainly monomer,and the expression of PKM2 tetramer is significantly reduced;Pretreatment TEPP-46 inhibited the invasion and proliferation of MGC-803 cells.Pretreatment of TEPP-46 promoted the expression of PKM2 tetramer and inhibited the expression of HIF-1αand p-NF-κB.Conclusion:TEPP-46 can inhibit the invasion and proliferation of gastric cancer cells by inhibiting PKM2 nuclear translocation,promoting PKM2 tetramer expression,and inhibiting HIF-1αand p-NF-κB expression.

关 键 词：胃肿瘤 TEPP-46 丙酮酸激酶2 侵袭 增殖 

分 类 号：R735.2[医药卫生—肿瘤]