4种香花型石斛花朵挥发性成分GC-MS分析  

GC-MS Analysis of Volatile Components in Flowers of Four Kinds of Fragrant Dendrobium Species

作　　者：曹桦[1] 许凤[1] 陆琳[1] 姬语潞 陈翔[2] 张宝琼 李涵[1] Cao Hua;Xu Feng;Lu Lin;Ji Yulu;Chen Xiang;Zhang Baoqiong;Li Han(Flower Research Institute,Yunnan Academy of Agricultural Sciences/National Research Center for Ornamental Horticulture Engineering,Kunming 650205;Fujian Forestry Science and Technology Experimental Center,Nanjing Fujian 363600;College of Tropical Crops,Yunnan Agricultural University,Pu’er Yunnan 665000)

机构地区：[1]云南省农业科学院花卉研究所/国家观赏园艺工程技术研究中心,昆明650205 [2]福建省林业科技试验中心,福建南靖363600 [3]云南农业大学热带作物学院,云南普洱665000

出　　处：《中国农学通报》2021年第13期56-62,共7页Chinese Agricultural Science Bulletin

基　　金：云南省科技厅重点研发计划“高原特色观赏园艺航天生物技术研发与产业化”(2018IB012);云南省科技厅重大科技专项计划“城市垂直绿化关键技术的研究与示范”(2018BB014);云南省科技人才与平台计划“技术创新人才培养对象项目李涵”(202005AD160014)。

摘　　要：旨在利用固相微萃取方法结合气相色谱-质谱联用仪(SPME/GC-MS),对香花型石斛花朵挥发性成分进行分析,为云南省香花型石斛兰种质资源筛选、新品种培育和石斛花精油产品的开发提供参考。通过SPME/GC-MS方法,对兜唇石斛、金钗石斛、铁皮石斛和美花石斛4种香花型石斛盛花期花朵的挥发性成分进行测定和分析。从4种石斛花朵中共鉴定出挥发性成分80种,所共有的挥发性成分为反式α-罗勒烯和月桂烯。其中,兜唇石斛的主要挥发性成分为乙酸异辛酯和反式-α-罗勒烯,相对含量为45.86%和37.3%;金钗石斛的主要挥发性成分为β-石竹烯,相对含量最高为20.7%;铁皮石斛的主要挥发性成分为α-蒎烯、柠檬烯和桉叶油醇,其相对含量分别为31.49%、14.6%和13.6%;美花石斛的主要挥发性成分为乙酸异辛酯,相对含量为83.21%。石斛花朵中的挥发性成分及相对含量具有明显差异,主要挥发性成分对其香味起决定性作用。The aim is to analyze the volatile components of Dendrobium fragrans flowers by solid phase microextraction combined with gas chromatography-mass spectrometry(SPME/GC-MS).The volatile components and their corresponding contents of Dendrobium aphyllum,D.nobile,D.candidum and D.loddigesii were determined by SPME/GC-MS.80 volatile components were identified from four Dendrobium species.The common volatile components of the four species were trans-α-ocimene andβ-myrcene.Among them,the main volatile components of D.aphyllum were 2-ethylhexyl acetate and trans-α-ocimene,with relative content of 45.86%and 37.3%,respectively;that of D.nobile wasβ-caryophyllene,with the highest relative content of 20.7%;the main volatile components of D.candidum wereα-pinene,limonene and cineole,with relative content of 31.49%,14.6%and 13.6%,respectively;the main volatile component of D.loddigesii was 2-ethylhexyl acetate,the relative content was 83.21%.The volatile components and relative contents of different kinds of Dendrobium flowers were different.These main volatile components play a decisive role in the fragrance of different Dendrobium flowers.

关 键 词：石斛属 香花型 挥发性成分 固相微萃取方法 气相色谱-质谱联用仪 

分 类 号：S682.31[农业科学—观赏园艺]