长链非编码RNA TSI抑制TGF⁃β1介导的乳腺癌细胞MCF⁃7和BT474转移  

Long non⁃coding RNA TSI inhibited the metastasis induced by TGF⁃β1 in breast cencer cell MCF⁃7 and BT474

作　　者：谭梓聪 张洋璠 黄晓燕 杨浩杰 曹铭辉[1,2] TAN Zi-cong;ZHANG Yang-fan;HUANG Xiao-yan;YANG Hao-jie;CAO Ming-hui(Department of Anesthesiology,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China;Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Reg-ulation,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China)

机构地区：[1]中山大学孙逸仙纪念医院麻醉科 [2]广东省恶性肿瘤表观遗传和基因调控重点实验室,广州510120

出　　处：《岭南现代临床外科》2021年第2期171-176,共6页Lingnan Modern Clinics in Surgery

基　　金：广州市科技计划项目(202002020002)。

摘　　要：目的研究长链非编码RNA TSI(Lnc-TSI)在TGF-β1促进乳腺癌细胞MCF-7和BT474转移中的作用。方法TGF-β1处理乳腺癌细胞MCF-7和BT474后,观察细胞形态变化,western blot实验检测上皮-间充质转化相关蛋白的变化,transwell实验检测细胞侵袭能力。qRT-PCR分别检测MCF-10A和TGF-β1处理前后MCF-7、BT474细胞的Lnc-TSI变化。在MCF-7和BT474细胞中分别敲除和过表达Lnc-TSI,transwell实验检测细胞在TGF-β1处理后侵袭迁移能力的变化。结果TGF-β1引起乳腺癌细胞MCF-7和BT474发生上皮-间充质转化,细胞形态呈梭形改变,上皮-间充质转化相关蛋白E-cadherin下调,N-cadherin和Vimentin上调,细胞侵袭数目增加。与正常乳腺上皮细胞MCF-10A细胞相比,MCF-7和BT474细胞中的Lnc-TSI表达更高。TGF-β1处理能显著上调MCF-7和BT474细胞中的Lnc-TSI水平。Transwell实验显示敲除和过表达Lnc-TSI能分别增强和抑制MCF-7和BT474细胞的侵袭和迁移,在TGF-β1处理下这种现象更为明显。结论在TGF-β1促进的乳腺癌细胞MCF-7和BT474的转移进程中Lnc-TSI表达上调,Lnc-TSI能抑制乳腺癌细胞MCF-7和BT474的转移。Objective To uncover the function of long non⁃coding RNA TSI(Lnc⁃TSI)in the TGF⁃β1 induced metastasis of breast cancer cell MCF⁃7 and BT474.Methods We treated the MCF⁃7 and BT474 cells with TGF⁃β1 and investigated the morphological changes.Epithelial⁃mesenchymal transition(EMT)associated proteins were measured with western blot and cell invasion were tested with transwell assay.And then qPCR was used toverify the expression of Lnc⁃TSI in MCF⁃10A cells,compared with MCF⁃7 and BT474 cells with or without TGF⁃β1 treatment.After knocking out and overexpressing Lnc⁃TSI in MCF⁃7 and BT474 cells respectively,we conducted transwell assay to see the changes of cell⁃migration and invasion.Results Morphology scale showed highly possible EMT changes in MCF⁃7 and BT474 cells.Also,EMT associated protein E⁃cadherin were down⁃regulated while N⁃cadherin and Vimentin were up⁃regulated,consisting with the enhanced invasion after treating with TGF⁃β1.Compared with MCF⁃10A cells,Lnc⁃TSI level was much higher in MCF⁃7 and BT474 cells.TGF⁃β1 dramatically up⁃regulated the expression of Lnc⁃TSI in MCF⁃7 and BT474 cells.Knocking out and overexpressing Lnc⁃TSI in MCF⁃7 and BT474 cells were able to enhanced and inhibited the cell migration and invasion respectively,which were both more obvious with TGF⁃β1 treatment.Conclusion Lnc⁃TSI was up⁃regulated following the metastasis mediated by TGF⁃β1 in MCF⁃7 and BT474 cells.Lnc⁃TSI suppressed the metastasis of MCF⁃7 and BT474 cells.

关 键 词：长链非编码RNA TGF⁃β1 乳腺癌 转移 

分 类 号：R737.9[医药卫生—肿瘤]