肝脏肿瘤体外培养模型的应用与研究进展  

Application and research progress of in vitro liver cancer cell culture models

作　　者：邢家利 王禹歆 杜顺达 Jia-Li Xing;Yu-Xin Wang;Shun-Da Du(Department of Liver Surgery,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]中国医学科学院北京协和医院肝脏外科,北京市100730

出　　处：《世界华人消化杂志》2021年第11期563-570,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金,No.81972698;湖北陈孝平科技发展基金会肝胆胰恶性肿瘤研究基金,No.CXPJJH11900001-2019215.

摘　　要：肝癌正逐渐成为世界各国公众健康的重要负担,而各种抗癌药物在肝癌的治疗中扮演着重要的作用,正确的选择药物,将显著改善患者预后.体外肝癌细胞培养模型是研究肝癌发病机制以及药物筛选的重要途径.长期的实践证明传统的二维药物筛选方法无法真实的再现肿瘤复杂的耐药机制,三维体外肝癌细胞模型的出现丰富了肝癌体外实验的方法选择的同时,在体外肝癌药物实验敏感性及肝癌细胞病理、生理学体外研究方面也带了较大提高.本文回顾了现有不同类型的肝癌细胞体外培养的主要类型、讨论其各自存在的优势及缺陷,以明确肝癌体外培养模型的发展和研究方向.Liver cancer is gradually becoming an important burden on public health around the world,and many drugs are currently available for the treatment of liver cancer,so the correct choice of drugs will significantly improve the prognosis of patients.In vitro liver cancer cell culture model is an important way to study the pathogenesis of liver cancer and drug screening.Long-term practice has proved that the traditional two-dimensional(2D)drug screening method cannot truly reproduce the complex drug resistance mechanism of tumor.The emergence of in vitro three-dimensional(3D)hepatocellular carcinoma cell model enriches the selection of methods for hepatoma experiments in vitro.The experimental sensitivity of hepatoma drugs in vitro and the study of pathology and physiology of hepatoma cells in vitro have also been greatly improved.In this paper,we review the main types of liver cancer cells cultured in vitro and discuss their advantages and disadvantages,in order to clarify the development and research direction of in vitro liver cancer culture models.

关 键 词：肝癌细胞来源 二维肝癌细胞培养 类器官 人源肿瘤异种移植模型 三维肝癌细胞培养 三维生物打印 

分 类 号：R735.7[医药卫生—肿瘤]