胶体金法检测粪便中TU M2PK、COX-2、Vimentin在大肠癌患者中的表达变化及临床意义分析  

Detection of TU M2PK,COX-2,and Vimentin expression in stool by colloidal gold method in patients with colorectal cancer and its clinical significance

作　　者：楼何飞 马春梅 傅英芝 张术 He-Fei Lou;Chun-Mei Ma;Ying-Zhi Fu;Shu Zhang(Clinical Laboratory of Pan’an Traditional Chinese Medical Hospital,Jinhua 322300,Zhejiang Province,China;The Affiliated Hospital of Hangzhou Normal University,Hangzhou 310015,Zhejiang Province,China)

机构地区：[1]磐安县中医院检验科,浙江省金华市322300 [2]杭州市第二人民医院,浙江省杭州市310015

出　　处：《世界华人消化杂志》2021年第11期601-608,共8页World Chinese Journal of Digestology

摘　　要：背景大肠癌一经发现大多都进入到中晚期,给治疗和术后恢复带来极大的困难.如何能在发病早期的不明显阶段发现病灶成为治疗的关键.胶体金法目前属于快速、简单、准确、经济的诊断方法,结合发病初期粪便中相关因子水平的变化,为大肠癌早期确诊提供依据.目的探讨胶体金法检测粪便中肿瘤M2型丙酮酸激酶(tumor M2-pyruvate kinase,TU M2PK)、环氧化酶-2(cyclooxygenase-2,COX-2)、波形蛋白(Vimentin)在大肠癌患者中的表达及临床意义.方法选取2013-01/2017-01我院124例大肠癌患者作为观察组,同期41例结肠镜检查阴性者作为对照组.对比两组及不同病理特征患者TU M2PK、COX-2、Vimentin阳性率,分析大肠癌患者生存情况相关因素.结果观察组TU M2PK、COX-2、Vimentin阳性表达率均高于对照组,差异有统计学意义(P<0.05);大肠癌患者TU M2PK、COX-2、Vimentin阳性表达在Dukes分期、分化程度方面相比,差异有统计学意义(P<0.05);不同Dukes分期、分化程度、TU M2PK、COX-2、Vimentin表达患者1年生存情况相比,差异有统计学意义(P<0.05);纳入Cox回归分析,调整其他混杂因素后,Dukes分期B期HR值为3.774;C期HR值为4.433;D期HR值为5.536;分化程度中分化HR值为0.414;高分化HR值为0.295;TU M2PK表达HR值为5.006;COX-2表达HR值为4.997;Vimentin表达HR值为4.671;TU M2PK、COX-2、Vimentin阳性表达患者1年生存率低于阴性患者,差异有统计学意义(P<0.05).结论大肠癌患者粪便中TU M2PK、COX-2、Vimentin异常表达,胶体金法检测上述指标对筛查大肠癌及评估患者生存情况具有潜在应用价值.BACKGROUND Colorectal cancer is often diagnosed at middle or late stage,which brings great difficulties to the treatment and postoperative recoverys.How to find the lesion at the early stage of the disease is the key to treatment.Colloidal gold method is a rapid,simple,accurate,and economical method for the early diagnosis of colorectal cancer.Combined with the changes of related factors in feces at the early stage of disease,it provides a basis for the early diagnosis of colorectal cancer.AIM To detect tumor M2 pyruvate kinase(TU M2PK),cyclooxy-genase-2(COX-2),and Vimentin expression in feces of patients with colorectal cancer by colloidal gold method and analyze its clinical significanced.METHODS From January 2013 to January 2017,124 patients with colorectal cancer diagnosed at our hospital were selected as an observation group,and 41 patients with negative colonoscopy during the same period were selected as a control group.The positive rates of TU M2PK,COX-2,and Vimentin between the two groups and patients with different pathological characteristics were compared,and factors related to the survival of patients with colorectal cancer were analyzed.RESULTS The positive expression rates of TU M2PK,COX-2,and Vimentin in the observation group were significantly higher than those in the control group(P<0.05).The positive expression of TU M2PK,COX-2,and Vimentin in patients with colorectal cancer were significantly different among patients with different Dukes stages and tumor differentiation degrees(P<0.05).There were statistically significant differences in the 1-year survival of patients with different Dukes stages,degrees of differentiation,and TU M2PK,COX-2,and Vimentin expression(P<0.05).After incorporating Cox regression analysis and adjusting for other confounding factors,the hazard ratio(HR)was 3.774 for Dukes stage B,4.433 for stage C,and 5.536 for stage D.The HR value was 0.414 for moderate differentiation and 0.295 for high differentiation;.The HR value was 5.006 for TU M2PK expression,4.997 for COX-2 exp

关 键 词：胶体金法 粪便 肿瘤M2型丙酮酸激酶 环氧化酶-2 波形蛋白 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]