Survival-associated alternative splicing events interact with the immune microenvironment in stomach adenocarcinoma  

作　　者：Zai-Sheng Ye Miao Zheng Qin-Ying Liu Yi Zeng Sheng-Hong Wei Yi Wang Zhi-Tao Lin Chen Shu Qiu-Hong Zheng Lu-Chuan Chen 

机构地区：[1]Department of Gastrointestinal Surgical Oncology,Fujian Cancer Hospital&Fujian Medical University Cancer Hospital,Fuzhou 350014,Fujian Province,China [2]Department of Clinical Laboratory,Fujian Maternity and Child Health Hospital,Affiliated Hospital of Fujian Medical University,Fuzhou 350001,Fujian Province,China [3]Department of Fujian Provincial Key Laboratory of Tumor Biotherapy,Fujian Cancer Hospital&Fujian Medical University Cancer Hospital,Fuzhou 350014,Fujian Province,China

出　　处：《World Journal of Gastroenterology》2021年第21期2871-2894,共24页世界胃肠病学杂志（英文版）

基　　金：the National Clinical Key Specialty Construction Program of China and Grants from the National Science Foundation Project of the Fujian Science and Technology Department,No.2017J01264 and No.2018Y0015;the Foundation for Fujian Provincial Health Technology Project,No.2019-ZQN-16,No.2019-CXB-9,and No.2019006;the Startup Fund for Scientific Research,Fujian Medical University,No.2017Q1219 and No.2017Q1220.

摘　　要：BACKGROUND Alternative splicing(AS)increases the diversity of mRNA during transcription;it might play a role in alteration of the immune microenvironment,which could influence the development of immunotherapeutic strategies against cancer.AIM To obtain the transcriptomic and clinical features and AS events in stomach adenocarcinoma(STAD)from the database.The overall survival data associated with AS events were used to construct a signature prognostic model for STAD.METHODS Differentially expressed immune-related genes were identified between subtypes on the basis of the prognostic model.In STAD,2042 overall-survival-related AS events were significantly enriched in various pathways and influenced several cellular functions.Furthermore,the network of splicing factors and overallsurvival-associated AS events indicated potential regulatory mechanisms underlying the AS events in STAD.RESULTS An eleven-AS-signature prognostic model(CD44|14986|ES,PPHLN1|21214|AT,RASSF4|11351|ES,KIAA1147|82046|AP,PPP2R5D|76200|ES,LOH12CR1|20507|ES,CDKN3|27569|AP,UBA52|48486|AD,CADPS|65499|AT,SRSF7|53276|RI,and WEE1|14328|AP)was constructed and significantly related to STAD overall survival,immune cells,and cancer-related pathways.The differentially expressed immune-related genes between the high-and low-risk score groups were significantly enriched in cancer-related pathways.CONCLUSION This study provided an AS-related prognostic model,potential mechanisms for AS,and alterations in the immune microenvironment(immune cells,genes,and pathways)for future research in STAD.

关 键 词：Stomach adenocarcinoma Alternative splicing Tumor microenvironment Immune-related genes and pathways 

分 类 号：R735.2[医药卫生—肿瘤]