肿瘤相关成纤维细胞与CD4^(+)、CD8^(+)T淋巴细胞在乳腺癌发生发展及淋巴结转移中的相关性研究  

Correlation of cancer-associated fibroblasts,CD4^(+) and CD8^(+) T lymphocytes with development and lymph node metastasis of breast carcinoma

作　　者：朱燕[1] 朱永祺 张晓娟[1] 钱金锋[1] 刘宏斌[1] 盛美红[3] ZHU Yan;ZHU Yongqi;ZHANG Xiaojuan;QIAN Jinfeng;LIU Hongbin;SHENG Meihong(Department of Pathology,the Second Affiliated Hospital of Nantong University,Nan tong 226001;Clinical Medicine of Medical College of Nantong University,Nantong 226001;Department of Image,the Second Affiliated Hospital of Nantong University,Nantong 226001,China)

机构地区：[1]南通大学第二附属医院病理科,江苏南通226001 [2]南通大学医学院临床医学系,江苏南通226001 [3]南通大学第二附属医院影像科,江苏南通226001

出　　处：《南京医科大学学报（自然科学版）》2021年第4期575-579,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南通市市级科技计划项目(JCZ18121);高层次卫生人才“六个一工程”拔尖人才科研项目(LGY2018036)。

摘　　要：目的:研究肿瘤相关性成纤维细胞(cancer-associated fibroblast,CAF)与CD4^(+)、CD8^(+)T淋巴细胞在乳腺癌发生发展及淋巴结转移中的相关性。方法:检测乳腺浸润性导管癌组织及癌旁组织中CAF相关蛋白α-平滑肌肌动蛋白(α-SMA)的表达和间质淋巴细胞CD4、CD8的表达,并结合临床病理特征进行统计学分析,从而探寻CAF诱导的免疫抑制与乳腺癌发生发展及淋巴结转移程度之间的相关性。结果:α-SMA蛋白在乳腺癌组织中呈高表达,并与肿瘤大小、临床分期及淋巴结转移呈正相关(P<0.05);CD4的阳性表达与年龄、肿瘤大小及临床分期无相关性(P>0.05),而与组织学分级及淋巴结转移呈负相关(P<0.05)。CD8的阳性表达与年龄、肿瘤大小及临床分期无相关性(P>0.05),而与组织学分级及淋巴结转移呈正相关(P<0.05)。经配对计数资料的相关分析,α-SMA蛋白的阳性表达与CD8阳性表达存在正相关(P<0.01),与CD4阳性表达存在负相关(P<0.01)。结论:CAF及上述免疫细胞的异常促进了乳腺癌发生发展及淋巴结转移,可为评估乳腺癌患者的生存及预后提供可靠的实验室依据。Objective:To study correlation of cancer-associated fibroblasts(CAFs),CD4^(+),CD8^(+) T lymphocyte with developments and lymph node metastasis of breast carcinoma.Methods:Immunohistochemistry method(S-P)was used to examine the expression ofα-smooth muscle actin(α-SMA),CD4 and CD8 in breast invasive ductal carcinoma,and the relationships with the clinicopathological features of breast carcinoma were analyzed to explore the correlation between CAFs induced immunosuppression and the development and lymph node metastasis of breast carcinoma.Results:The positive rate ofα-SMA was significantly higher in breast carcinoma than that in control.The expression rate ofα-SMA was positively correlated to the tumor size,TNM stage and lymph nodal involvement(P<0.05).The expression of CD4 was negatively correlated to the grade and lymph nodal involvement(P<0.05).The expression of CD8 was positively correlated to the grade and lymph nodal involvement(P<0.05).The expression of α-SMA was positively correlated to the expression of CD8(P<0.01),and it was negatively correlated to the expression of CD4(P<0.01).Conclusion:Abnormal CAFs and CD4^(+),CD8^(+) T lymphocytes promote the occurrence,development and lymphatic metastasis of breast cancer,and they provide reliable laboratory basis to evaluate the survival and prognosis of breast cancer patients.

关 键 词：CAF CD4 CD8 乳腺浸润性导管癌 淋巴结转移 

分 类 号：R737.9[医药卫生—肿瘤]