增加CaSR表达减轻2型糖尿病大鼠心肌纤维化的机制  

Mechanism of increased CaSR expression in alleviating myocardial fibrosis in type 2 diabetic rats

作　　者：徐娜[1] 王跃虹[1] 姜晓姝[1] 徐长庆[1] 白淑芝[1] XU Na;WANG Yue-hong;JIANG Xiao-shu;XU Chang-qing;BAI Shu-zhi(Department of Pathophysiology,Harbin Medical University,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学病理生理学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2021年第1期7-10,共4页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金资助项目(H2017007)。

摘　　要：目的观察钙敏感受体(CaSR)对糖尿病心肌病大鼠心肌纤维化的影响。方法Wistar大鼠随机分为对照组、糖尿病组和精胺干预组(n=10)。通过高糖高脂饮食喂养联合腹腔注射STZ(30 mg/kg)建立糖尿病模型。监测各组大鼠饮食、饮水的改变,透射电镜观察各组大鼠心肌超微结构的改变,超声心动仪检测心脏功能,Western blot检测心肌组织CaSR、Smad蛋白的表达。结果与对照组相比,糖尿病组大鼠饮食饮水明显增加,心脏功能降低,心肌CaSR蛋白表达降低,Smad蛋白表达升高,心肌超微结构显示纤维化损伤严重,给予精胺干预后明显逆转。结论精胺可能通过增加CaSR表达、降低Smad蛋白减轻2型糖尿病大鼠心肌纤维化,改善心脏功能。Objective To observe the effect of CaSR on myocardial fibrosis in diabetic cardiomyopathy rats.Methods Wistar rats were randomly divided into control group,diabetic group and spermine intervention group(n=10).Diabetes model was established by high sugar and high fat diet combined with intraperitoneal injection of STZ(30 mg/kg).Changes in diet and drinking water of rats in each group were monitored,changes in myocardial ultrastructure were observed by transmission electron microscopy,cardiac function was detected by echocardiography,and expressions of CaSR and Smad proteins in myocardial tissue were detected by Western blot.Results Compared with the control group,rats in the diabetic group had signifi-cantly increased diet and drinking water,decreased cardiac function,decreased myocardial CaSR protein expression,increased Smad protein expression,and myocardial ultrastructure showed serious fibrosis damage,which was significantly reversed after arginine intervention.Conclusion Spermine may reduce myocardial fibrosis in type 2 diabetic rats and improve cardiac function by increasing CaSR expression and decreasing Smad protein.

关 键 词：精胺 糖尿病 钙敏感受体 心肌纤维化 

分 类 号：R363[医药卫生—病理学]