培养基配方和培养条件对大杯蕈孢子萌发的影响  

Effects of Medium Formula and Culture Conditions on Spore Germination of Pleurotus giganteus

作　　者：陈雪凤[1] 吴圣进[1] 韦仕岩[1] 潘庆流 张雯龙[1] 刘增亮[1] CHEN Xuefeng;WU Shengjin;WEI Shiyan;PAN Qingliu;ZHANG Wenlong;LIU Zengliang(Institute of Microbiology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;Guangxi Yizhou Agricultural Science Research Institute,Yizhou,Guangxi 546300)

机构地区：[1]广西农业科学院微生物研究所,广西南宁530007 [2]广西宜州市农业科学研究所,广西宜州546300

出　　处：《北方园艺》2021年第10期117-123,共7页Northern Horticulture

基　　金：广西农业科学院基本科研业务专项资助项目(桂农科2020YM75,桂农科2018YT18);广西宜州桑枝食用菌试验站资助项目(桂TS2016021);广西农科院科技先锋队专项资助项目(桂农科JZ202011);国家食用菌产业技术体系广西创新团队建设专项资金资助项目(nycytxgxcxtd-07-02)。

摘　　要：以大杯蕈孢子为试材,采用平板涂布法,对影响其孢子萌发的培养基、温度、酸碱度(pH)、金属离子、三十烷醇浓度及光照条件进行研究,以期筛选出孢子萌发的最适条件,为大杯蕈遗传育种中单孢菌株的获得提供有效方法和参考依据。结果表明:大杯蕈孢子最适萌发条件为使用20%马铃薯+2%蛋白胨固体培养基、20%马铃薯+2%可溶性淀粉固体培养基或2%蛋白胨固体培养基;最适萌发温度为30℃;最适酸碱度为pH 5~6;最适金属离子为ZnSO4;最适三十烷醇浓度为0.050 mg·(100mL)^(-1);黑暗条件利于孢子萌发。Pleurotus giganteus was used as test material.Several factors as for different medium,temperature,pH,metal ion,concentrations of triacontanol and light conditions were tested to analyse effects of the spores germination of Pleurotus giganteus.In order to screen out the optimal conditions for spore germination and provide favorable conditions for single spore strain obtaining in Pleurotus giganteus genetic breeding.The results showed that the optimum medium was 20% potato+2%peptone,20% potato+2% soluble starch and 2% peptone.The optimum temperature was 30 ℃.The optimum pH was 5-6.The optimal metal ion was ZnSO4.The optimum concentration of triacontanol was 0.050 mg·(100 mL)^(-1).Dark condition were favorable for spore germination.

关 键 词：大杯蕈 孢子萌发 影响因子 

分 类 号：S646[农业科学—蔬菜学]