CYP11B2基因-344C/T位点多态性 在云南彝族人群中的分布  

CYP11B2 Gene-344C/T Polymorphism in Yunnan Yi Population

作　　者：彭红瑜 范志祥[1] 张延洁[1] 舒芯 张馥麟 龙莉[1] Peng Hongyu;Fan Zhixiang;Zhang Yanjie(School of Basic Medicine,Kunming Medical University,Yunnan 650000,China)

机构地区：[1]昆明医科大学,650000

出　　处：《医学研究杂志》2021年第5期121-124,共4页Journal of Medical Research

摘　　要：目的研究云南省彝族人群CYP11B2基因-344C/T位点多态性的分布情况,分析其多态分布是否存在民族和种族特异性。方法采集286例云南省晋宁县双河乡彝族居民外周血DNA,采用PCR-RFLP技术特异扩增目的片段,限制性内切酶HaeⅢ酶切后产生不同大小片段以鉴定基因型,计算基因型和等位基因频率,并与其他人群进行比较。结果彝族人群中CYP11B2基因-344C/T位点基因型频率分别为TT(59.79%),CT(33.92%)和CC(6.29%),等位基因T/C频率为76.75%和23.25%,与不同民族、国家人群比较,除汉族与韩国人群外,不同人群此位点多态分布比较差异有统计学意义(P<0.05)。结论CYP11B2基因-344C/T位点多态性分布存在民族和种族差异。Objective To investigate the distribution of CYP11B2 gene-344C/T polymorphism in Yi population of Yunnan Province,explore the ethnic specificity.Methods Totally 286 cases of cross-blood DNA from Yi population in Shuanghe Township,Jinning County,Yunnan Province were collected,and PCR-RFLP was used to specifically expand the target fragments.The fragments of different length were generated after digestion with endonuclease HaeⅢ.Genotypes and allele frequencies were calculated and compared with other populations.Results In Yi minority,CYP11B2(-344C/T)genotype frequencies for the TT,CT and CC were 59.79%,33.92%,6.29%,and the allele frequencies of T/C were 76.75%,23.25%respectively.Except Han population and Korean,significant differences in the distribution of CYP11B2(-344C/T)genotypes and the allele frequencies were observed between the people in Yi population and other different minorities or countries(P<0.05).Conclusion The CYP11B2(-344C/T)polymorphism has national and racial differences.

关 键 词：CYP11B2基因 基因多态性 彝族 

分 类 号：R181[医药卫生—流行病学]