帕金森病模型大鼠纹状体^(11)C-CFT microPET/CT多巴胺转运蛋白显像与中脑黑质多巴胺能神经元受损的相关性研究  

Correlation between ^(11)C-CFT microPET/CT dopamine transporter imaging of striatum in Parkinson′s disease model rats and damage to dopamine neurons in the substantia nigra of midbrain

作　　者：黄世明 岳建兰 尹亮 孙永锋[2] 林志春 张锦明[3] Huang Shiming;Yue Jianlan;Yin Liang;Sun Yongfeng;Lin Zhichun;Zhang Jinming(Department of Nuclear Medicine,Featured Medical Center of Chinese People′s Armed Police Forces,Tianjin 300162,China;Military Medicine and Special Discipline,Beijing Armed Police Corps Hospital,Beijing 100027,China;Department of Nuclear Medicine,the First Medical Center,Chinese PLA General Hospital,Beijing 100039,China)

机构地区：[1]武警特色医学中心核医学科,天津300162 [2]武警北京总队医院军事医学与特种学科,100027 [3]解放军总医院第一医学中心核医学科,北京100039

出　　处：《中华核医学与分子影像杂志》2021年第5期291-295,共5页Chinese Journal of Nuclear Medicine and Molecular Imaging

摘　　要：目的探讨^(11)C-2β-甲基酯-3β-(4-氟苯基)托烷(CFT)microPET/CT多巴胺转运蛋白显像与中脑黑质多巴胺能神经元损伤程度及帕金森病(PD)严重程度的相关性。方法60只雄性Sprague-Dawley(SD)大鼠按随机数字表法分为PD模型组(n=48)和对照组(n=12),通过6-羟基多巴胺(6-OHDA)注入右侧纹状体建立PD模型,分别在建立PD模型后1、2、3、4周进行旋转行为测试、^(11)C-CFT microPET/CT显像,计算损伤侧/健侧纹状体^(11)C-CFT摄取比值;通过免疫荧光染色分别计数双侧黑质致密部中酪氨酸羟化酶(TH)免疫阳性神经元的数量,并计算损伤侧/健侧的比值。对数据进行单因素方差分析、最小显著差异t检验和Pearson相关分析。结果PD模型建立后1、2、3、4周,PD模型向健侧旋转的旋转速度依次为(4.55±1.37)、(8.64±1.64)、(9.96±1.83)、(11.67±2.77)r/min,而对照组均未出现任何旋转行为;PD模型组^(11)C-CFT摄取比值与TH阳性神经元数量比值分别为0.658±0.038、0.580±0.094、0.513±0.042、0.394±0.065与0.698±0.066、0.604±0.062、0.546±0.064、0.315±0.082,均明显低于对照组(0.997±0.048与0.996±0.054;F值:167.50、169.20,均P<0.05)。^(11)C-CFT摄取比值与旋转行为学结果(旋转速度)、TH阳性神经元比值结果均具有相关性(r值:-0.877、0.897,均P<0.001)。结论PD动物模型中,^(11)C-CFT摄取比值与中脑黑质多巴胺能神经元损伤程度(TH阳性神经元比值)以及PD严重程度有很好的相关性。Objective To explore the correlations between ^(11)C-2β-carbomethoxy-3β-(4-fluorophenyl)tropane(CFT)microPET/CT imaging and the degree of damage to dopamine neurons in the substantia nigra of midbrain and the severity of Parkinson′s disease(PD).Methods Sixty male Sprague-Dawley(SD)rats were divided into PD model group(n=48)and control group(n=12)by random number table method.The PD model was established by injecting 6-hydroxydopamine(6-OHDA)into the right striatum.The rotational behavior test and ^(11)C-CFT microPET/CT imaging were performed at 1,2,3 and 4 weeks after the establishment of PD model.The radioactivity uptake values of bilateral striatum were analyzed and the radioactivity uptake ratio of injured side to healthy side was calculated.The number of tyrosine hydroxylase(TH)immunoreactive positive neurons in the pars compacta of substantia nigra was counted by immunofluorescence staining,and the ratio of total number of TH positive neurons in injured side to healthy side was calculated.Data were analyzed by one-way analysis of variance,the least significant difference t test and Pearson correlation analysis.Results At 1,2,3 and 4 weeks after the establishment of PD model,the rotation speed of PD model to the healthy side was(4.55±1.37),(8.64±1.64),(9.96±1.83)and(11.67±2.77)r/min,respectively,while there was no rotation behavior in the control group.Meanwhile,the ratios of ^(11)C-CFT uptake and the number of TH positive neurons in the PD model group were 0.658±0.038,0.580±0.094,0.513±0.042,0.394±0.065 and 0.698±0.066,0.604±0.062,0.546±0.064,0.315±0.082,respectively,which were significantly lower than those in the control group(0.997±0.048 and 0.996±0.054;F values:167.50,169.20,both P<0.05).Correlation analysis showed that ^(11)C-CFT uptake ratio was correlated with rotation behavior(rotation speed)and TH positive neuron ratio(r values:-0.877,0.897,both P<0.001).Conclusion In the PD animal model,the ratios of ^(11)C-CFT uptake has a good correlation with the degree of damage to dopamine ne

关 键 词：帕金森病 纹状体 多巴胺质膜转运蛋白质类 中脑 多巴胺能神经元 正电子发射断层显像术 体层摄取术 X线计算机 托烷类 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]