溃疡性结肠炎患者中TLR9和STAT3的表达及其与IL-10的相关性研究  

Expressions of TLR9 and STAT3 and their correlation with IL-10 in patients with ulcerative colitis

作　　者：张雯婷 顾林[1] 柯希权 王启之[1] 汪强武[1] 汪建超[1] 李大鹏[1] ZHANG Wen-ting;GU Lin;KE Xi-quan;WANG Qi-zhi;WANG Qiang-wu;WANG Jian-chao;LI Da-peng(Department of Gastroenterology,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China)

机构地区：[1]蚌埠医学院第一附属医院消化内科,安徽蚌埠233004

出　　处：《中华全科医学》2021年第6期921-924,共4页Chinese Journal of General Practice

基　　金：安徽省教育厅2018年度高校优秀拔尖人才培育资助项目(gxgwfx2018052);蚌埠医学院自然科学重点项目(BYKY2019085ZD)。

摘　　要：目的检测Toll样受体9(TLR9)、信号转导与转录活化因子3(STAT3)及白介素10(IL-10)在溃疡性结肠炎(ulcerative colitis, UC)患者外周血及肠黏膜组织中的表达水平,探讨它们在UC发病中的作用及其相互关系。方法选取2019年7月—2020年7月在蚌埠医学院第一附属医院诊治的32例UC患者归为实验组,30例健康体检者纳入正常对照组,其中实验组又分为轻度(10例)、中度(14例)及重度组(8例)。检测外周血单个核细胞中TLR9 mRNA和STAT3 mRNA的表达和血清IL-10水平及肠黏膜组织中TLR9、STAT3及IL-10蛋白表达水平。结果实验组UC患者TLR9 mRNA和STAT3 mRNA的表达水平均高于正常对照组(均P<0.05);血清中IL-10的表达水平明显低于正常对照组(P<0.05)。肠黏膜组织中TLR9、STAT3的蛋白表达水平均高于正常对照组(均P<0.05),IL-10蛋白表达水平低于正常对照组(P<0.05)。TLR9 mRNA和STAT3 mRNA、TLR9和STAT3蛋白表达水平之间均呈正相关,血清IL-10与TLR9 mRNA、STAT3 mRNA表达水平呈负相关,肠黏膜组织IL-10与TLR9、STAT3蛋白表达水平呈负相关。结论 TLR9、STAT3、IL-10共同参与了UC的发生和发展,在UC的发病机制中TLR9和STAT3可能参与了IL-10的表达与调控。Objective The expression levels of toll-like receptor 9(TLR9), signal transduction and transcriptional activator factor 3(STAT3) and interleukin-10(IL-10) in peripheral blood and intestinal mucosal tissues of patients with ulcerative colitis(UC) were detected to explore their roles in the pathogenesis of UC and their correlation. Methods Thirty-two patients with UC treated in the First Affiliated Hospital of Bengbu Medical College from July 2019 to July 2020 were selected as the experimental group;and 30 healthy subjects according to the results of physical examination were included in the normal control group. The experimental group was further divided into mild group(n=10), moderate group(n=14) and severe(n=8) group. The expressions of TLR9 and STAT3 mRNA in peripheral blood mononuclear cells, serum IL-10 levels and TLR9, as well as levels of STAT3 and IL-10 proteins, in intestinal mucosal tissues were detected. Results The expression levels of TLR9 mRNA and STAT3 mRNA in the experimental group were both higher than those in the control group(all P<0.05). The expression level of IL-10 in serum in the experimental group was significantly lower than that in the normal control group(P<0.05). The expression levels of TLR9 and STAT3 in intestinal mucosal tissues were all higher than that in the normal control group(all P<0.05). By contrast, the expression levels of IL-10 in the experimental group were lower than that in the normal control group(P<0.05). Expressions of TLR9 mRNA and STAT3 mRNA and TLR9 and STAT3 mRNA were positively correlated. Serum IL-10 was negatively correlated with TLR9 and STAT3 mRNA expression levels. Intestinal mucosal IL-10 was negatively correlated with TLR9 and STAT3 protein expression levels. Conclusion TLR9, STAT3 and IL-10 are jointly involved in the occurrence and development of UC. TLR9 and STAT3 may be involved in the expression and regulation of IL-10 in the pathogenesis of UC.

关 键 词：溃疡性结肠炎 TOLL样受体9 信号转导与转录活化因子3 白介素10 

分 类 号：R574.62[医药卫生—消化系统] R446[医药卫生—内科学]