基于数据挖掘的马齿苋治疗非酒精性脂肪肝机制研究  

Mechanism of Portulaca oleracea L. in the treatment of non-alcoholic fatty liver disease based on data analysis

作　　者：王鑫[1] 吴巧敏 汤雨潇 张美芳[1] 陈洁文[1] WANG Xin;WU Qiao-min;TANG Yu-xiao;ZHANG Mei-fang;CHEN Jie-wen(Department of Clinical Nutrition,the Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201900,China;不详)

机构地区：[1]上海交通大学医学院附属第九人民医院临床营养科,上海201900 [2]海军军医大学海军医学系营养与食品卫生学教研室,上海200433

出　　处：《中华全科医学》2021年第6期950-953,共4页Chinese Journal of General Practice

基　　金：国家自然科学基金青年基金(81903306)。

摘　　要：目的探讨马齿苋治疗非酒精性脂肪肝(non-alcoholic fatty liver disease, NAFLD)的分子基础与作用机制。方法通过TCMSP、Drug Bank、Uniprot等数据库,筛选出马齿苋的有效活性成分及其作用靶点。利用Drugbank、OMMI、GeneCards等数据库找出与NAFLD相关的基因靶点,最后取两者交集。最后利用DAVID数据库进行KEGG和GO富集分析。结果通过筛选得出马齿苋有10种有效成分,其中槲皮素、山奈酚、木犀草素、β-谷固醇、β-胡萝卜素是主要活性成分。共得到85个关键交集靶点,其中肿瘤坏死因子(TNF)、半胱氨酸天冬氨酸蛋白酶(CASP)、Ser/Thr蛋白激酶(AKT1)、炎症趋化因子(CCL2)为重要靶点。DAVID富集分析显示,GO功能分析主要参与调控炎症反应(P<0.001)、细胞缺氧(P<0.001)、凋亡(P<0.001)、相似蛋白质结合(P<0.001)、蛋白质二聚化活性(P<0.001)、细胞因子活性(P<0.001)等方面。KEGG富集分析主要与TNF(P<0.001)、细胞凋亡(P<0.001)、TLR(P<0.001)、NF-κB(P<0.001)等信号通路有关。结论本研究通过初步探讨马齿苋治疗NAFLD的潜在靶点和相关信号通路,为进一步深入揭示其作用机制及开发马齿苋功能性食品提供了理论依据。Objective To explore the molecular mechanism of Portulaca oleracea L. in the treatment of non-alcoholic fatty liver(NAFLD). Methods With the use of the TCMSP and Drug Bank and Uniprot database, the effective activity of Portulaca oleracea L. was screened out ingredients and their potential targets. The Drug bank, OMMI and GeneCards databases were used in identifying gene targets related to NAFLD and intersection targets. Finally, the DAVID database was used in conducting KEGG and GO enrichment analyses for determining the potential signal pathways of Portulaca oleracea L. for NAFLD treatment. Results Portulaca oleracea L. had 10 effective active ingredients, of which quercetin, kaempferol, luteolin, β-sitosterol and β-carotene were the main active ingredients. A total of 85 key targets were identified after matching with NAFLD gene targets, and tumor necrosis factor(TNF), cysteine aspartic protease, Ser/Thr protein kinase and inflammatory chemokines were found to be important targets. DAVID enrichment analysis showed that GO functional analysis were mainly involved in inflammation(P<0.001), cell hypoxia(P<0.001), and apoptosis(P<0.001), identical protein binding(P<0.001), protein polymerisation activity(P<0.001) and cytokine activity(P<0.001). KEGG enrichment analysis mainly related to TNF(P<0.001), apoptosis(P<0.001), TLR(P<0.001) and NF-κB signal pathway(P<0.001). Conclusion This study preliminarily explores the potential targets and related signal pathways of Portulaca oleracea L. in NAFLD treatment and provides a theoretical basis for further revealing mechanisms for developing the functional food of Portulaca oleracea L.

关 键 词：马齿苋 非酒精性脂肪肝 网络药理学 

分 类 号：R575.5[医药卫生—消化系统] R96[医药卫生—内科学]