载银锌介孔钙硅纳米粒子根管密封性能的研究  

A study of the sealing properties of silver and zinc incorporated mesoporous calcium-silicate nanoparticles as a canal sealer

作　　者：李铭铭 周杨 冷迪雅 王莹[1] 吴大明[1] 李谨[3] LI Mingming;ZHOU Yang;LENG Diya;WANG Ying;WU Daming;LI Jin(Jiangsu Key Laboratory of Oral Diseases,Nanjing Medical University,Department of Endodontics,the Afiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院牙体牙髓病科,江苏南京210029 [2]南京医科大学姑苏学院,南京医科大学附属苏州医院,苏州市立医院口腔科,江苏苏州215002 [3]南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院特诊科,江苏南京210029

出　　处：《口腔医学》2021年第5期413-417,共5页Stomatology

基　　金：东南大学-南京医科大学合作研究项目(2018DN0015)。

摘　　要：目的评价载银锌介孔钙硅纳米粒子(silver and zinc incorporated mesoporous calcium-silicate nanoparticles, Ag-Zn-MCSNs)作为根管封闭剂的理化性能及根管密封性能。方法测定MCSNs、Ag-MCSNs、Zn-MCSNs、Ag-Zn-MCSNs(Ag∶Zn=1∶1,物质的量比)、AH-Plus糊剂的pH值、溶解度及流动性。单根恒牙根管预备后,以MCSNs、Ag-MCSNs、Zn-MCSNs、Ag-Zn-MCSNs、AH-Plus作为根管封闭剂,热牙胶技术充填根管。利用粪肠球菌(Enterococcus faecalis,E.faecalis)(ATCC 29212)渗漏法评估根管密封性能,Micro-CT扫描评估根管充填的空隙率。结果 4种纳米材料糊剂均为强碱性,溶解度小,具有良好的流动性(P<0.05);Ag-MCSNs组的渗漏样本最少(25%),其次是Ag-Zn-MCSNs和AH-Plus组(35%),MCSNs组最多(50%)(P>0.05);实验组的平均空隙率为2.09%,根尖段空隙率最高,其次是根中段和根上段(P<0.05)。结论 MCSNs糊剂具有良好的流动性,Ag-MCSNs和Ag-Zn-MCSNs显著减少E.faecalis渗漏,根管密封性能良好。Objective To evaluate the physicochemical and sealing properties of silver and zinc incorporated mesoporous calcium-silicate nanoparticles(Ag-Zn-MCSNs) as a canal sealer. Methods The pH values, solubility and flow values of MCSNs, Ag-MCSNs, Zn-MCSNs, Ag-Zn-MCSNs(Ag∶Zn=1∶1, molar ratio) and AH-Plus were measured. MCSNs, Ag-MCSNs, Zn-MCSNs, Ag-Zn-MCSNs and AH-Plus were used as canal sealers and prepared permanent teeth of single-canal root were obturated using warm vertical compaction technique. Enterococcus faecails(ATCC 29212) leakage test was used to evaluate the sealing properties and Micro-CT was used to calculate the percentage of voids within canals. Results The solution of four groups was strongly alkaline. They had lower solubility and higher flow values(P<0.05). Ag-MCSNs group had least leaked samples(25%), followed by Ag-Zn-MCSNs and AH-Plus groups(35%), while MCSNs group had most leaked samples(50%)(P>0.05). All experimental groups showed low ratios of voids, 2.09% on average, after obturation(P>0.05), which had the largest values in the apical sections, followed by the middle and coronal sections(P<0.05). Conclusion MCSNs paste possesses good fluidity and Ag-MCSNs, Ag-Zn-MCSNs could reduce E. faecalis leakage, which indicates good sealing properties.

关 键 词：介孔钙硅纳米粒子 根管封闭剂 细菌渗漏 根管密封性 

分 类 号：R783.1[医药卫生—口腔医学]