硝呋齐特通过miR-877-5p/蛋白磷酸酶2A的癌性抑制因子通路调控肺癌细胞增殖、迁移和侵袭的研究  

Nifuratezide regulates the proliferation,migration and invasion of lung cancer cells through miR-877-5p/protein phosphatase 2A cancer suppressor pathway

作　　者：纳建荣[1] 丁学梅[1] 马宣[1] 周玮[1] NA Jian-rong;DING Xue-mei;MA Xuan;ZHOU Wei(Department of Respiratory and Critical Diseases,General Hospital of Ningxia Medical University,Yinchuan 750002,Ningxia Hui Autonomous Region,China)

机构地区：[1]宁夏医科大学总医院呼吸与危重症学科,宁夏回族自治区银川750002

出　　处：《中国临床药理学杂志》2021年第11期1375-1379,共5页The Chinese Journal of Clinical Pharmacology

基　　金：心血管病疾病国家重点实验室项目(2017kf-02)。

摘　　要：目的探讨硝呋齐特对肺癌细胞增殖、迁移和侵袭的影响及作用机制。方法实验分为对照组(不做任何处理)、低、中、高剂量实验组(5,10和20μmol·L^(-1)硝呋齐特)、miR-NC组(转染miR-NC)、miR-877-5p组(转染miR-877-5p mimics)、anti-miR-NC-20组(转染anti-miR-NC+20μmol·L^(-1)硝呋齐特)和+anti-miR-877-5p-20组(转染anti-miR-877-5p+20μmol·L^(-1)硝呋齐特)。用噻唑蓝检测细胞增殖抑制率,用Transwell检测细胞迁移和侵袭,用逆转录聚合酶链反应法检测miR-877-5p和蛋白磷酸酶2A的癌性抑制因子(CIP2A)mRNA表达水平。结果对照组、低、高剂量实验组、miR-NC组、miR-877-5p组、anti-miR-NC-20组和anti-miR-877-5p-20组细胞增殖抑制率分别为(0.00±0.01)%,(14.65±1.42)%,(54.23±5.71)%,(5.47±0.58)%,(34.58±3.44)%,(54.31±5.48)%和(19.65±1.87)%,细胞迁移数分别为(124.33±11.07),(98.67±9.17),(61.22±6.18),(125.44±10.24),(69.33±6.67),(62.78±6.34)和(101.56±9.51)个,细胞侵袭数分别为(109.33±10.07),(81.33±8.35),(52.33±5.14),(106.33±9.44),(59.56±5.87),(53.44±5.96)和(89.56±8.43)个,miR-877-5p相对表达水平分别为1.01±0.09,1.52±0.15,2.64±0.25,1.00±0.08,2.54±0.25,2.69±0.25,1.53±0.15,CIP2A mRNA分别为1.02±0.08,0.86±0.07,0.54±0.05,1.01±0.06,0.62±0.05,0.51±0.04和0.79±0.05。低、高剂量实验组的上述指标与对照组比较,miR-877-5p组的上述指标与miR-NC组比较,anti-miR-877-5p-20组的上述指标与anti-miR-NC-20组比较,差异均有统计学意义(均P<0.05)。结论硝呋齐特可抑制肺癌细胞A549增殖、迁移和侵袭,其机制可能与miR-877-5p和CIP2A有关。Objective To investigate the effects and mechanisms of nifurate on the proliferation,migration and invasion of lung cancer cells.Methods The experiment was divided into control group(no treatment),experimental-L,-M,-H groups(5,10 and 20μmol·L^(-1) nifurazide),and miR-NC group(transfected miR-NC),miR-877-5 p group(transfected with miR-877-5 p mimics),antimiR-NC-20 group(transfected with anti-miR-NC+20μmol·L^(-1) nifurazide)and anti-miR-877-5 p-20 group(transfected with anti-miR-877-5 p+20μmol·L^(-1) nifurazide).Tetramethylazozolium colorimetry was used to detect the inhibition rate of cell proliferation.Transwell was used to detect cell migration and invasion,and reverse transcription polymerase chain reaction was used to detect the expression levels of miR-877-5 p and cancerous inhibitor of protein phosphatase 2 A(CIP2 A)mRNA.Results The cell proliferation inhibition rates of control group,experimental-L,-M,-H groups,miR-NC group,miR-877-5 p group,anti-miR-NC-20 group,anti-miR-877-5 p-20 group were(0.00±0.01)%,(14.65±1.42)%,(54.23±5.71)%,(5.47±0.58)%,(34.58±3.44)%,(54.31±5.48)%,(19.65±1.87)%,the number of cell migration was(124.33±11.07),(98.67±9.17),(61.22±6.18),(125.44±10.24),(69.33±6.67),(62.78±6.34)and(101.56±9.51),the number of cell invasion was(109.33±10.07),(81.33±8.35),(52.33±5.14),(106.33±9.44),(59.56±5.87),(53.44±5.96)and(89.56±8.43),the relative expression levels of miR-877-5 p were1.01±0.09,1.52±0.15,2.64±0.25,1.00±0.08,2.54±0.25,2.69±0.25 and 1.53±0.15,the relative expression levels of CIP2 A mRNA were 1.02±0.08,0.86±0.07,0.54±0.05,1.01±0.06,0.62±0.05,0.51±0.04 and 0.79±0.05.There were statistically significant differences between experimental-L,-H groups and control group,between miR-877-5 p group and miR-NC group,between anti-miR-877-5 p-20 group and anti-miR-NC-20 group(all P<0.05).Conclusion Nifuroxazide can inhibit the proliferation,migration and invasion of lung cancer cell A549,and its mechanism may be related to miR-877-5 p and CIP2 A.

关 键 词：硝呋齐特 miR-877-5p 蛋白磷酸酶2A的癌性抑制因子 肺癌 增殖 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]