Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection  

作　　者：Jingui Deng Yujing Huang Qing Wang Jianming Li Yanping Ma Ying Qi Zhongyang Liu Yibo Li Qiang Ruan 

机构地区：[1]Virology Laboratory,The Affiliated Shengjing Hospital,China Medical University,Shenyang 110004,China [2]Department of Laboratory,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China [3]Department of Gynecology and Obstetrics,The Affiliated Shengjing Hospital,China Medical University,Shenyang 110004,China [4]Department of Obstetrics,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China

出　　处：《Virologica Sinica》2021年第2期241-253,共13页中国病毒学（英文版）

基　　金：supported by the National Natural Science Foundation of China (81672028 and 81371788)。

摘　　要：Human cytomegalovirus(HCMV)is a double-strand DNA virus widely infected in human.Circular RNAs(circ RNAs)are non-coding RNAs with most functions of which keep unknown,and the effects of HCMV productive infection on host circ RNA transcriptions remain unclear.In this study,we profiled 283 host circ RNAs that significantly altered by HCMV productive infection in human embryonic lung fibroblasts(HELF)by RNA deep sequencing and bioinformatics analysis.Among these,circ SP100,circ MAP3 K1,circ PLEKHM1,and circ TRIO were validated for their transcriptions and sequences.Furthermore,characteristics of circ SP100 were investigated by RT-q PCR and northern blot.It was implied that circ SP100 was produced from the sense strand of the SP100 gene containing six exons.Kinetics of circ SP100 and SP100 m RNA were significantly different after infection:circ SP100 levels increased gradually along with infection,whereas SP100 m RNA levels increased in the beginning and dropped at 24 h post-infection(hpi).Meanwhile,a total number of 257 proteins,including 10 HCMV encoding proteins,were identified potentially binding to cytoplasmic circ SP100 by RNA antisense purification(RAP)and mass spectrometry.Enrichment analysis showed these proteins were mainly involved in the spliceosome,protein processing,ribosome,and phagosome pathways,suggesting multiple functions of circ SP100 during HCMV infection.

关 键 词：Human cytomegalovirus(HCMV) Productive infection Circular RNA(circRNA) circ SP100 TRANSCRIPTION 

分 类 号：R511[医药卫生—内科学]