基于熵权法和正交设计优化加减当归芍药散挥发油的β-环糊精包合工艺  

Optimization ofβ-cyclodextrin inclusion process of modified Danggui Shaoyao San volatile oil based on entropy method and orthogonal design

作　　者：成余勤 石森林[1] 来平凡[1] CHENG Yu-qin;SHI Sen-lin;LAI Ping-fan(College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州310053

出　　处：《中草药》2021年第10期2951-2957,共7页Chinese Traditional and Herbal Drugs

基　　金：缓控释给药系统研究室“组分中药(中药复方现代化)制剂研究中心”立项资助(ZFZX202002)。

摘　　要：目的优化β-环糊精包合加减当归芍药散(modified Danggui Shaoyao San,MDSS)中挥发油的最佳工艺。方法采用熵权法确定包合物得率及挥发油包合率的权重系数,以综合评分为指标,β-环糊精与挥发油的比例、包合温度、包合时间作为包合工艺的关键参数,采用正交试验优化包合工艺,通过薄层色谱、紫外光谱、红外光谱、X射线衍射、扫描电镜对包合物进行表征。结果挥发油包合的较优工艺为挥发油与β-环糊精(1∶10)在包合温度为40℃的条件下,包合2 h。经验证,包合物得率为64.56%,挥发油包合率为80.44%。挥发油包入β-环糊精空腔中,包合物制备成功。结论该工艺可用于MDSS挥发油的包合,可为后续深入研究奠定基础。Objective To optimize theβ-cyclodextrin inclusion process for volatile oil of modified Danggui Shaoyao San(MDSS).Methods Taking the ratio ofβ-cyclodextrin to volatile oil,inclusion temperature,and stirring time as the factors,the inclusion process conditions were optimized by orthogonal test.The weight coefficients of inclusion compound yield and volatile oil inclusion rate were determined by entropy weight method,and the inclusion complex was verified by thin layer chromatography,UV spectroscopy and infrared spectroscopy.The inclusion compounds were characterized by thin-layer chromatography,ultraviolet spectroscopy,infrared spectroscopy,X-ray diffraction,and scanning electron microscopy.Results The optimal conditions were determined to be 10:1 forβ-cyclodextrin-volatile oil ratio,40℃for in clusion temperature,and 2 h for stirring time,the inclusion rate and yield were 80.44%and 64.56%,respectively.The inclusion of volatile oils intoβ-cyclodextrin indicated that inclusion compounds were successfully prepared.Conclusion This stable and feasible method can be used for the inclusion of volatile oils of MDSS and it can lay the foundation for follow-up in-depth research.

关 键 词：加减当归芍药散 挥发油 Β-环糊精 熵权法 正交试验 包合工艺 

分 类 号：R283.6[医药卫生—中药学]