间充质干细胞移植改善脑室周围白质软化损伤作用的研究  

Study on the Effect of Mesenchymal Stem Cell Transplantation on Softening Damage of Periventricular White Matter

作　　者：巴依尔才次克[1] 王彦梅 朱艳萍[1] BA Yiercaicike;WANG Yanmei;ZHU Yanping(Department of Neonatology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院新生儿科,乌鲁木齐830054

出　　处：《实验动物科学》2021年第2期22-29,共8页Laboratory Animal Science

基　　金：新疆维吾尔自治区自然科学基金面上项目《干细胞移植对新生大鼠脑室周围白质软化的修复作用研究》(编号:2016D01C271)。

摘　　要：目的探讨间充质干细胞移植侧脑室后对缺血缺氧所致的脑室周围白质软化病理损伤的改善作用及相关机制。方法7日龄SD大鼠构建脑白质损伤模型,72只鼠随机分成三组:空白组、模型组和移植组。右侧颈总动脉结扎后缺氧处理2.5 h。造模24 h之后,采用脑立体定位注射法将间充质干细胞注入移植组侧脑室。并于7 d和14 d后探究实验动物学习记忆功能变化、病理损伤、细胞凋亡及干细胞增殖水平变化。结果间充质干细胞能显著抑制颈总动脉结扎所致的细胞损伤及凋亡,促进损伤部位干细胞增殖,髓鞘形成增加,学习记忆功能得到改善。结论间充质干细胞移植后能在损伤部位能促进神经干细胞的增殖和分化,进而对缺血缺氧所致的脑室周围白质软化病理损伤产生修复作用。Objective The purpose of this study was to investigate the effect of mesenchymal stem cells transplanted into the lateral ventricle on the pathological damage of periventricular white matter softening caused by ischemia and hypoxia,and to analyze the possible mechanism.Method Seven-day-old SD rats were used to construct the model of periventricular leukomalacia.First,72 rats were randomly divided into 3 groups:control group,model group and hUC-MSC transplantation group.After that,the rats in the model group and transplantation group were ligated with the right common carotid artery and treated with hypoxia for 2.5 hours.After 24 hours of modeling,mesenchymal stem cells were injected into the lateral ventricle of the transplantation group by stereotactic brain injection.The changes of learning and memory function,pathological injury,cell apoptosis and stem cell proliferation in experimental animals were investigated 7 and 14 days later.Result Mesenchymal stem cells can significantly inhibit pathological injury,reduce the level of cell apoptosis,promote the proliferation of stem cells at the site of injury and myelin formation,and thus improve the learning and memory function.Conclusion After transplantation,mesenchymal stem cells can promote the proliferation and differentiation of neural stem cells at the injury site,and then repair the pathological damage of periventricular leukomalacia caused by ischemia and hypoxia.

关 键 词：髓鞘脱失 脑室周围白质软化 细胞凋亡 人脐带间充质干细胞 

分 类 号：R-332[医药卫生]