马铃薯空心块茎转录组分析  

Transcriptome Analysis of Hollow Heart Potato

作　　者：张引弟[1,2] 陈爱娥 白继鹏 王波[1,2] 王洪洋 李灿辉[1,2] Zhang Yindi;Chen Aie;Bai Jipeng;Wang Bo;Wang Hongyang;Li Canhui(Yunnan Key Laboratory of Potato Biology,Yunnan Normal University,Kunming,650500;Key Lab of Potato Biology in Universities of Yunnan Province,Yunnan Normal University,Kunming,650500;Teaching Affairs Office,Yunnan Normal University,Kunming,650500)

机构地区：[1]云南师范大学,云南省马铃薯生物学重点实验室,昆明650500 [2]云南师范大学,云南省高校马铃薯生物学重点实验室,昆明650500 [3]云南师范大学教务处,昆明650500

出　　处：《分子植物育种》2021年第10期3185-3195,共11页Molecular Plant Breeding

基　　金：国家自然科学基金(31800134,32060499);云南省应用基础研究计划面上项目(2019FB023)共同资助。

摘　　要：空心是马铃薯块茎内部生理缺陷性状,它会严重影响马铃薯的鲜食和加工。为了解马铃薯空心块茎不同形成时期的差异表达基因和探究与空心块茎形成密切相关功能基因。本研究采用RNA-Seq技术对马铃薯空心敏感性材料(’5-19’)块茎无空心期(N)、空心轻微期(S,空心长度0~1 cm)、空心较重期(M,空心长度1~3 cm)进行转录组分析。结果显示,各送样样品高质量碱基均达到6.54 Gb,各组样品Q30碱基百分比均在94.15%以上。N-vs-S和S-vs-M的差异表达基因(differentially expressed genes,DEGs)数目分别为44和130个。GO功能注释显示差异表达基因主要集中在代谢过程、细胞过程、单组织过程、结合、催化活性、细胞组分等。KEGG代谢通路富集发现,差异表达基因显著富集到角质素、木栓质、蜡状物合成、苯丙烷生物合成、脂肪酸延长、苯丙氨酸代谢等途径。进一步分析挖掘到19个与马铃薯块茎空心形成相关基因。本研究解析了马铃薯空心块茎形成过程中的转录调控分子机制,为后续研究提供了相关基因数据信息。Hollow heart is internal defective traits of potato,which seriously affect the processing and fresh of potatoes.In order to study the gene expression difference during the different stages of hollow heart potato tuber and some genes involved in regulating hollow heart were excavated.RNA-Seq technology was used to analyze the changes of hollow heart potato tubers from sensitive material(’5-19’)during no hollow stage(N,hollow length 0 cm),slight hollow stage(S,hollow length 0~1 cm),moderate hollow stage(M,hollow length 1~3 cm)in this study.The results showed that each sample obtained more than 6.54 Gb clean bases,and the percentage of Q30 bases in each sample was more than 94.15%.The number of differentially expressed genes(DEGs)during N-vs-S and S-vs-M were 44 and 130 genes,respectively.GO analysis showed that these DEGs were mainly referred to metabolic process,cellular process,catalytic activity,binding,single organism process.KEGG pathway analysis showed that these DEGs were mainly referred to cutin,suberine and wax biosynthesis,phenylpropanoid biosynthesis,fatty acid elongation,and phenylalanine metabolism.A further analysis showed that 19 genes were closely related to the formation of hollow heart.These results were partly explained molecular mechanism of the hollow heart formation of potato and provided valuable information on relevant genetic data for subsequent studies.

关 键 词：马铃薯 空心 转录组 差异表达基因 

分 类 号：S532[农业科学—作物学]