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作 者:Yangning OU Chenghao ZHANG Xiumei MA Jiangcun WEI Wen ZHONG Lihua YU Yusheng HUANG Lizhen CAO
机构地区:[1]Hezhou Hospital of Traditional Chinese Medicine, Hezhou 542899, China [2]Guangxi International Zhuang Medicine Hospital, Nanning 530201, China
出 处:《Medicinal Plant》2021年第2期21-23,29,共4页药用植物:英文版
基 金:Scientific Research and Technological Development Program Project of Hezhou City(He Ke Ji 20047);Scientific Research Program of Guangxi International Zhuang Medicine Hospital in 2020(GZ202001);Preparation Quality Improvement Project of Traditional Chinese Medicine Zhuang and Yao Medicine Hospital of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region(GZZJ202013&GZZJ202015);Zhuang and Yao Medicine Research Laboratory of Guangxi International Zhuang Medicine Hospital.
摘 要:[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase:acetonitrile-0.1%glacial acetic acid;flow rate:1.0 mL/min;column temperature:30℃;detection wavelength:360 nm;sample injection volume:10μL.[Results]The kaempferol content in Yao medicine L.foenum-graecum was determined,Y=20652X-151098(R=0.9997)shows a good linear relationship within the linear range of 9.80-156.8μg/mL.[Conclusions]The high performance liquid chromatography(HPLC)method was established for determining the kaempferol content in Yao medicine L.foenum-graecum.This method is expected to provide a scientific basis for the formulation of quality control methods for L.foenum-graecum.
关 键 词:Lysimachia foenum-graecum Hance. KAEMPFEROL High performance liquid chromatography(HPLC)
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