Longan Aril Reverses H2O2 Cytotoxicity in PC12 Cells via RAS/MEK/ERK Signaling Pathway  

作　　者：Tianrong LEI Yang CAO Chang LI Jianghua ZHANG Honghe XIAO Cheng SONG Hongyan LI 

机构地区：[1]Pharmaceutical College, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China [2]Dalian Huaxin Physicochemical Testing Centre Ltd., Dalian 116600, China [3]College of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China [4]Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China

出　　处：《Medicinal Plant》2021年第2期54-59,共6页药用植物：英文版

基　　金：Liaoning Natural Science Foundation(20180530033);Open Fund of Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Liaoning University of Traditional Chinese Medicine。

摘　　要：[Objectives]To explore the neuroprotective effects and mechanism of Longan Aril(LA)effective parts on PC12 cells injured by H2O2.[Methods]The neuroprotective effects of LA were evaluated by the cell viability,SOD and MDA content,apoptosis assay and relative protein expression of Aβand p-Tau.The neuroprotective mechanism of LA was studied by using metabolomics and network pharmacology,and the expressions of RAS/MEK/ERK signaling pathway-related proteins were detected by western blotting.[Results]LA could improve the cell survival rate and SOD content,and reduce apoptosis and expression of Aβand p-tau.Inhibition of RAS/MEK/ERK signaling pathway is a possible mechanism of LA neuroprotective effects.[Conclusions]LA has a neuroprotective effects in vitro and be likely to inhibit the process of AD by inhibition of RAS/MEK/ERK signalling pathway.

关 键 词：Longan Aril(LA) RAS/MEK/ERK pathway Alzheimer's disease(AD) Neuroprotective effects 

分 类 号：R73[医药卫生—肿瘤]