Determination of Polydatin in Rat Serum and Its Changes by UPLC Method  

作　　者：Haisheng ZENG Zhiqiang WANG Meiyuan LU Chao ZENG Mengxia YANG Guilin YANG 

机构地区：[1]The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China [2]The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China [3]Guangxi Food and Drug Evaluation & Inspection Center, Nanning 530029, China

出　　处：《Medicinal Plant》2021年第2期63-64,69,共3页药用植物：英文版

基　　金：Project of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region(GZZC2019147);Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2020(2020KY13034);The First Batch High-level Talent Scientific Research Project of The Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(Y20196311);National Traditional Chinese Medicine Characteristic Technology Heritage Talent Training Program(2015481601003);Program of Youjiang Medical University for Nationalities(yy2018ky018).

摘　　要：[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the analytical column,acetonitrile-water(55∶45)was used as the mobile phase,the flow rate was 0.5 mL/min,and the column temperature was 30℃and the detection wavelength was 306 nm.[Results]The linear range of the established serum sample analysis method was 1.0-20.0μg/mL,and the correlation coefficient was r=0.9994;the intraday and interday RSD of Wistar rat serum was less than 3.0%,and the accuracy was higher than 90%.[Conclusions]This method is sensitive,accurate,and rapid.It is suitable for monitoring the concentration of polydatin in serum after intragastric administration,and can also be used for pharmacokinetics and bioavailability studies.

关 键 词：POLYDATIN Drug concentration in serum Content determination Ultra Performance Liquid Chromatography(UPLC) 

分 类 号：R28[医药卫生—中药学]