淫羊藿苷对RSV感染诱发哮喘小鼠体内PGD2水平的影响  

The Effect of Icariin on the Prostaglandin D2 Level in Asthmatic Mice Induced by Respiratory Syncytial Virus Infection

作　　者：袁丽粉[1] 孙淑宁[1] 张扬扬 乔建瓯[1] 庞淯阳 YUAN Li-fen;SUNShu-ning;ZHANG Yang-yang;QIAO Jian-ou;PANG Yu-yang(Departments of Respiratory and Critical Care Medicine,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China;Departments of Hematology,Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院呼吸与危重症医学科,上海200011 [2]上海交通大学医学院附属第九人民医院血液内科,上海200011

出　　处：《现代生物医学进展》2021年第10期1806-1810,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81170028)。

摘　　要：目的:观察淫羊藿苷对RSV感染诱发哮喘小鼠血清及支气管肺泡灌洗液中前列腺素D2(Prostaglandin D2,PGD2)表达水平的影响,以期为哮喘治疗寻找新的靶点.方法:30只Balb/c小鼠随机均分为三组:即正常组,OVA/RSV-YYH组(即淫羊藿苷治疗组)及OVA/RSV-非YYH组(即未经淫羊藿苷治疗组).卵蛋白致敏RSV感染诱发小鼠哮喘模型成功建立后,予以淫羊藿苷2.5 mg连续腹腔注射治疗2周,比较治疗前后肺功能检测结果、支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中细胞分类计数、血清及BALF中PGD2表达水平、肺组织病理学变化.结果:淫羊藿苷治疗后,哮喘小鼠肺功能较治疗前明显改善,差异有统计学意义(P<0.05);PGD2水平较前明显下降,差异有统计学意义(P<0.05);各分类白细胞计数较前明显减少,差异有统计学意义(P<0.01),气道管壁增厚及管腔狭窄现象较前明显改善,肺组织炎症细胞浸润较前减少.结论:淫羊藿苷可有效降低RSV感染诱发哮喘小鼠体内炎性介质PGD2水平,从而改善气道重塑,减轻小鼠的哮喘症状,它可能是以后哮喘治疗的一个新的靶点.Objective:To observe the effect of icariin on the expression of prostaglandin D2(PGD2)in serum and bronchoalveolar lavage fluid(BALF)of asthmatic mice.We hoped to find a new target for the treatment of asthma.Methods:Thirty Balb/c mice were randomly divided into three groups:normal group,OVA/RSV-YYH group(icariin treatment group)and OVA/RSV-non-YYH group(without icariin treatment group).After establishing the mouse model of asthma which is sensitized by ovalbumin(OVA)and induced by respiratory syncytial virus(RSV)infection,we treated the mice with an intraperitoneal injection of 2.5 mg icariin continually for two weeks.The results of pulmonary function tests,cell classification and count in BALF,the PGD2 level in serum and BALF and pathologic changes of lung tissues before and after the treatment were compared respectively.Results:Compared with those without the treatment of icariin,lung function of asthmatic mice was improved significantly,the difference was statistically significant(P<0.05);PGD2 level was decreased,the difference was statistically significant(P<0.05);the leukocyte count was obviously decreased,the difference was statistically significant(P<0.05);the thickened wall and narrow lumen of the airways were better,and infiltration of inflammatory cells in lung tissues was evidently relieved.Conclusion:Icariin could effectively reduce PGD2 level of asthmatic mice which were induced by RSV infection,hence relieve airway remodeling and asthmatic symptoms of mice.PGD2 might be a new target for asthma treatment in the future.

关 键 词：淫羊藿苷 哮喘 呼吸道合胞病毒 前列腺素D2 卵蛋白 

分 类 号：R-33[医药卫生] R562.25