德普昌达克冰川和中山站抗生素抗性基因及影响因素研究  

Antibiotic resistance genes and their influencing factors in Depuchangdake Glacier and Zhongshan Station

作　　者：张淑红 何瑞 侯书贵[2] 刘科 李治国 ZHANG Shuhong;HE Rui;HOU Shugui;LIU Ke;LI Zhiguo(College of Biology and Food,Shangqiu Normal University,Shangqiu 476000,China;College of Geography and Oceanography,Nanjing University,Nanjing 210093,China;College of Surveying and Planning,Shangqiu Normal University,Shangqiu 476000,China)

机构地区：[1]商丘师范学院生物与食品学院,河南商丘476000 [2]南京大学地理与海洋学院,江苏南京210093 [3]商丘师范学院测绘与规划学院,河南商丘476000

出　　处：《环境保护科学》2021年第3期151-158,共8页Environmental Protection Science

基　　金：国家自然科学基金重点项目(41830644);国家自然科学基金面上基金项目(41671072);河南省自然科学基金资助项目(182300410137)。

摘　　要：为了解冰川环境抗生素抗性基因(Antibiotic Resistance Genes,ARGs)的存在状况及其与重金属和I型整合子(class I integron,intI)的关系,通过荧光定量PCR(Real-time Quantitative PCR,qPCR),研究青藏高原德普昌达克冰川和南极中山站雪样ARGs、重金属抗性基因(heavy metaI resistance genes,HMRGs)和intI的相对丰度及其相关性。结果表明,大部分基因的相对丰度在德普昌达克冰川小于中山站。德普昌达克冰川tetA、czcD、pcoA、znuA、intI1和intI2的相对丰度随海拔高度的增加而降低;南极的merA、zntA、znuA、sul2、tetA和intI2距离中山站建筑区域越近,相对丰度越高。2个研究位点ARGs均受Zn抗性基因znuA的影响,且znuA与intI之间显著相关。冰川环境虽然远离人类活动,但其ARGs同时受intI和与人类活动有关的重金属Zn的影响。To investigate the occurrence of the antibiotic resistance genes(ARGs)and the relationship between the ARGs and the heavy metals or type I integron(intI)in the glacial environment,the Real-time Quantitative PCR(qPCR)was used to analyze the relative abundance of the ARGs,heavy metal resistance genes(HMRGs)and intI,as well as their correlation in the glacial snow samples from Depuchangdake Glacier on the Qinghai-Tibet Plateau and Zhongshan Station in Antarctica.The results showed that the relative abundance of most genes detected in Depuchangdake Glacier was lower than that in Zhongshan Station.The relative abundance of tetA,czcD,pcoA,znuA,intI1 and intI2 in Depuchangdake Glacier decreased with the increasing of the altitude.The relative abundance of merA,zntA,znuA,sul2,tetA and intI2 were higher with a closer distance to the building area in Zhongshan Station.ARGs in the 2 sites were affected by Zn resistance gene znuA,which was significantly and positively correlated with intI.Thus,ARGs in glacial environments were affected by both intI and Zn,which was closely related to human activities.

关 键 词：抗生素抗性基因 重金属抗性基因 I型整合子 德普昌达克 中山站 

分 类 号：X171[环境科学与工程—环境科学]