北京某医院儿童肺炎支原体肺炎患者血清非靶向代谢组学分析  

Untargeted metabolomic analysis of serum samples from children with mycoplasma pneumonia in a hospital in Beijing

作　　者：惠秦 张琪[1] 王琨蒂 张静 郑承宁 周忠蜀 Hui Qin;Zhang Qi;Wang Kundi;Zhang Jing;Zheng Chengning;Zhou Zhongshu(Graduate School of Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100070,China;Department of Pediatrics,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]中国医学科学院北京协和医学院研究生院,100070 [2]中日友好医院儿科,北京100029

出　　处：《中华预防医学杂志》2021年第5期660-666,共7页Chinese Journal of Preventive Medicine

基　　金：中国医学科学院医学与健康科技创新工程项目 (2018-I2M-1-003)。

摘　　要：目的分析北京某医院儿童肺炎支原体肺炎患者血清非靶向代谢组学。方法选取于2019年1月至2020年2月在中日友好医院儿科住院诊治的儿童肺炎支原体肺炎患者为病例组,同时选取年龄、性别匹配的健康体检儿童为对照组。最终纳入100例研究对象,病例组和对照组分别为50例、50名。采集研究对象晨起空腹静脉血2 ml,采用超高效液相色谱质谱法测定血清中代谢产物,采用无监督的主成分分析及有监督的(正交)偏最小二乘法分析相结合的方法找到组间的差异代谢物,并通过MBRole进行通路富集分析。结果50例病例组研究对象(男、女童分别为27、23例),年龄为(6.00±3.65)岁;50名对照组研究对象(男、女童分别为28、22名)年龄为(6.62±2.64)岁。共检测出392种差异代谢物,与对照组相比,病例组降低的有306种,升高的有86种。VIP>5且P值<0.05的差异代谢物有41种,主要为磷脂类物质,与对照组相比,病例组降低的有38种,升高的有3种。通路富集分析结果显示,代谢通路包括苯丙氨酸、酪氨酸、色氨酸、不饱和脂肪酸、氨酰tRNA的生物合成,胰岛素信号途径及ABC转运蛋白的代谢。结论儿童肺炎支原体肺炎患者与正常儿童的血清非靶向代谢组存在差异。Objective This study was aimed to analyze the untargeted metabolomics of serum samples from children with mycoplasma pneumonia in a hospital in Beijing.Methods A total of 50 children with mycoplasma pneumonia as the case group were recruited from Department of Pediatrics,China-Japan Friendship Hospital in Beijing from January 2019 to February 2020,and meanwhile 50 age-and gender-matched heathy children were selected and formed the control group.2 ml venous fasting blood samples was collected from all children.Serum metabolites were quantified by using the untargeted ultra-high performance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS)technique.Unsupervised principle component analysis and(orthogonal)partial least-squares-discriminant analysis were employed to identify differential metabolites between cases and controls.MBRole software was used for pathway enrichment analysis.Results There were 27 boys and 23 girls in the case group with an average age of(6.0±3.65)years,and the control group consisted of 28 boys and 22 girls with an average age of(6.62±2.64)years.A total of 392 different metabolites were detected.Compared with the control group,306 metabolites were decreased and 86 increased in case group.Forty-one differential metabolites with variable important in projection(VIP)values larger than 5 and P values less than 0.05 were teased out,and they mainly concentrated on phospholipid.The levels of 38 metabolites were significantly lower in the case group,yet 4 metabolites were significantly higher than that of the control group.Metabolic enrichment analysis showed that different metabolites were related to the biosynthesis of phenylalanine,tyrosine,tryptophan,unsaturated fatty acid,ammonia acyl tRNA and insulin signaling pathway,as well as the metabolism of ABC transporters.Conclusion The serum untargeted metabolomics differed remarkably between children with mycoplasma pneumonia and healthy children.

关 键 词：中日友好医院 超高效液相色谱质谱法 偏最小二乘法 性别匹配 不饱和脂肪酸 代谢通路 磷脂类 ABC转运蛋白 

分 类 号：R725.6[医药卫生—儿科]