Ⅰ型登革病毒在中国的传播及进化  

Transmission and evolution of Dengue virus type Ⅰ in China

作　　者：李代英 鲁明 朱海莲 何丽存 顾冉 张娟 陈俊英[1,2] 潘玥 孙强明[1,2] LI Dai-ying;LU Ming;ZHU Hai-lian;HE Li-cun;GU Ran;ZHANG Juan;CHEN Jun-ying;PAN Yue;SUN Qiang-ming(Institute of Medical Biology,Chinese Acculemy of Medical Science&Peking Union Medical College,Yunnan Key Lalioratory of Vaccine Research&Development on Severe Infections Diseases,Kunming 650118,Yunrum Province,China)

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室,云南昆明650118 [2]云南省虫媒传染病防控研究重点实验室,云南普洱665000

出　　处：《中国生物制品学杂志》2021年第5期522-527,共6页Chinese Journal of Biologicals

基　　金：国家自然科学基金面上项目(31970868);云南省重大专项和重点研发计划(2019ZF004);中国医学科学院创新工程项目(2016-I2M-1-19);云南省医学领军人才项目(L-2019030)。

摘　　要：目的研究近年来中国境内登革病毒(Dengue virus,DENV)流行株分布与东南亚株的联系,分析传播规律,追溯传入源头,探讨其系统发育和分子进化特征。方法以公共卫生科学数据中心公布的病例数分析国内Ⅰ型DENV(Dengue virus-1,DENV-1)感染及分布情况,收集NCBI中自1985年来在中国境内流行的405株DENV-1毒株全基因序列,使用在线工具MAFFT进行序列对比,并通过ATGC网站在线PhyML构建进化树。基于进化树分析结果挑选40余个代表性毒株与东南亚代表株序列,使用MEGA X软件进行系统进化树分析;随后对中国DENV-1流行株序列进行重组进化分析。结果自2004—2017年间国内共有66 623人感染登革热,其中广东省每年均有DENV-1病例报道,自2012年以来,DENV-1引起的病例数呈现了迅速增加的情况,地域范围不断扩大。国内流行株与东南亚国家流行株存在密切联系,不断出现输入病例,同时也存在广东省向东南亚地区输出DENV-1的可能。云南省存在由边境从中南半岛地区国家传入DENV流行株,并之后在云南长期流行的现象。云南省输入毒株与广东省输入的DENV毒株差异较大,可认为其DENV毒株输入来源不同。24个代表株的重组分析结果表明,虽然2017年广东株MN018297部分序列与2014年广东株KP723476相似,但未发现更多的重组现象。结论通过将中国境内DENV-1毒株汇总后进行系统进化树的构建,分析并挑选出代表性株后进行分析,发现了DENV毒株传入中国主要经由的省份,并结合氨基酸位点的比对分析DENV在中国境内流行分支,为研究DENV-1在中国的传播和进化提供了参考。Objective To investigate the association of distribution of epidemic strain of Dengue virus(DENV)in China with the isolates in Southeast Asia,analyze the regularity of transmission,trace the source and explore the phylogenesis and molecular evolutionary characteristics of the virus. Methods Based on the number of cases published by the Public Health Science Data Center,the infection and distribution of Dengue virus type I(DENV-1)in China were analyzed,and the complete gene sequences of 405 DENV-1 strains in NCBI database since 1985 were collected,of which the sequences were compared by using the online tool MAFFT,and a phylogenetic tree was constructed by using online phyML on ATGC website. Based on the results of phylogenetic analysis, more than 40 representative strains and and the representative strains from Southeast Asia were selected and subjected to phylogenetic analysis by using MEGA X software. Afterwards,the sequences of epidemic DENV-1 strains in China were subjected to recombinant evolutionary analysis. Results From 2004 to 2017,a total of 66 623 cases of dengue fever were reported in China,among which the cases caused by DENV-1 were reported in Guangdong Province every year. Since 2012,the number of cases caused by DENV-1 increased rapidly,and the geographical scope was also expanded. The epidemic strains in China were closely associated with those in Southeast Asia. The imported cases increased continuously,and there was also possible export of DENV-1 from Guangdong Province to Southeast Asia. In Yunnan Province,epidemic DENV strains were introduced from the countries of Indochina Peninsula and then spread in Yunnan for a long time. Significant difference was observed between the imported DENV strains in Yunnan Province and Guangdong Province,which may be considered that the imported strains came from different sources. The results of recombination analysis of 24 representative strains showed that though the partial sequence of Guangdong strain MN018297 in 2017 was similar to that of Guangdong strai

关 键 词：登革病毒 登革热 传播 系统进化分析 

分 类 号：R373.3[医药卫生—病原生物学]