不同干预治疗对PCOS大鼠血管生成素样蛋白2表达的影响  

Effects of Different Interventions on the Expression of Angiopoietin Like Protein 2 in PCOS Rats

作　　者：王丹丹 柴淑娟[1] 郭秋炎 卫项龙 郭艺红 申科[1] 李艳春[1] 赵瑞钦 WANG Dandan;CHAI Shujuan;GUO Qiuyan(The Third Affiliated Hospital of He’nan University of TCM,Zhengzhou City,He’nan Province450008;不详)

机构地区：[1]河南中医药大学第三附属医院,河南省郑州市450008 [2]舞阳县妇幼保健院生殖科 [3]郑州大学第一附属医院

出　　处：《医学理论与实践》2021年第12期1989-1991,2008,共4页The Journal of Medical Theory and Practice

摘　　要：目的:观察补肾化瘀方与二甲双胍联合应用对多囊卵巢综合征(PCOS)模型大鼠血管生成素样受体2表达的干预作用,探讨PCOS的部分作用机制。方法:选60只21d龄雌性SD大鼠,设立空白对照组10只,其余50只大鼠采用来曲唑灌胃建立PCOS大鼠模型。成模大鼠随机分为5组:模型对照组、西药组、中药低浓度组、中药高浓度组、联合用药组,每组10只。分别进行相应药物干预21d。采用ELISA法检测各组大鼠血清胰岛素含量,采用RT-PCR法检测大鼠卵巢组织中血管生成素样蛋白2(ANGPTL2)、AKT、Foxol mRAN水平,采用Westen-blot法检测大鼠卵巢组织中ANGPTL2、Foxol、AKT蛋白表达水平。结果:与空白对照组相比,模型组大鼠血清胰岛素及卵巢组织中ANGPTL2、AKT、Foxol mRNA及蛋白表达水平明显升高,差异有显著性(P<0.05);与模型对照组相比,中西医联合治疗组血清胰岛素水平显著下降,差异有显著性(P<0.05),而西药组在降低ANGPTL2及凋亡相关因子AKT、Foxol mRNA及蛋白表达水平效果优于中西药联合治疗组。结论:补肾化瘀方与二甲双胍联合应用能够显著改善PCOS大鼠模型卵巢组织中胰岛素抵抗,但该中药方剂有可能会促进血管生成导致ANGPTL2的生成增加。Objective:To observe the effect of the combination of Bushen Huayu prescription and metformin on the expression of angiopoietin-like receptor 2 in polycystic ovary model rats,and to explore the partial mechanism of PCOS.Methods:606-week-old female SD rats were selected,10 were set up in the blank control group,and the remaining 50 rats were fed with letrozole to establish the model of PCOS rats.Model rats were randomly divided into 5 groups:model control group,western medicine group,low concentration group of traditional Chinese medicine,high concentration group of traditional Chinese medicine,combined use group,10 in each group.Respectively,the corresponding drug intervention was carried out for 21 days.ELISA was used to detect the serum insulin content in each group of rats.RT-PCR was used to detect the levels of angiopoietin-like protein 2,AKT,Foxol mRNA in rat ovary tissue.We used westen-blot to detect the expression levels of angiogenin-like protein 2,Foxol,and AKT protein in rat ovary tissue.Results:Compared with the blank control group,serum insulin and angiopoietin like protein 2,Foxol and AKT in ovarian tissue of model group were significantly higher than those in control group.Compared with the model control group,the serum insulin level in the combined treatment group was significantly decreased(P<0.05),while the effect of Western medicine group in reducing the expression of angiopoietin like protein 2 and apoptosis related factors AKT,Foxol mRNA and protein was better than that of the combined treatment group.Conclusion:The combination of Bushen Huayu prescription and metformin can significantly improve insulin resistance in the ovarian tissue of PCOS rat model.The traditional Chinese medicine prescription may promote angiogenesis and increase the production of angiopoietin-like protein 2.

关 键 词：多囊卵巢综合征 血管生成素样蛋白2 二甲双胍 

分 类 号：R-33[医药卫生]