uc.12+A在B细胞淋巴瘤中的功能研究  

Function of uc.12+A in B lymphoma cells

作　　者：张欣 华婧 刘金晶 杨哲 郭俊荣 戴华 张艳青 ZHANG Xin;HUA Jing;LIU Jinjing;YANG Zhe;GUO Junrong;DAI Hua;ZHANG Yanqing(Jiangsu Key Laboratory of Experimental&Translational Non-Coding RNA Research/College of Medicine,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学医学院/江苏省非编码RNA基础与临床转化重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2021年第2期57-62,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金面上项目(81670186);江苏省高校自然科学研究重大项目(20KJA320005);扬州大学研究生学术科技创新基金项目(XKYCX19-154)。

摘　　要：从C57BL/6野生型小鼠骨髓中分选出正常B细胞,应用实时荧光定量PCR (qRT-PCR)检测uc.12+A在小鼠正常B细胞和B淋巴瘤细胞的表达水平。通过分子克隆将uc.12+A基因序列构建到pMSCV-PIG反转录病毒表达载体上,包装成反转录病毒感染小鼠B淋巴瘤细胞38B9和人Burkitt淋巴瘤细胞Romas,经嘌呤霉素筛选后形成稳定过表达uc.12+A的B淋巴瘤细胞系uc.12+A-38B9和uc.12+A-Romas。流式细胞术检测细胞凋亡和周期,体外细胞计数比较细胞增殖情况,小鼠皮下荷瘤测量肿瘤重量。结果表明:与小鼠正常B细胞相比,uc.12+A在小鼠B淋巴瘤细胞中的表达水平增高。这一研究提示过表达uc.12+A显著促进B淋巴瘤细胞的增殖,减少其凋亡并增加B细胞淋巴瘤的成瘤速率。Normal B cells were sorted from the bone marrow of C57 BL/6 wild-type mice, and real-time fluorescent quantitative PCR(qRT-PCR) was used to detect the expression level of uc.12+A in normal mouse B cells and B lymphoma cells. The uc.12+A gene sequence was constructed on the pMSCV-PIG retrovirus expression vector by molecular cloning, and packaged into retrovirus to infect mouse B lymphoma cell 38 B9 and human Burkitt lymphoma cell Romas. After selection by puromycin, the B lymphoma cell lines uc.12+A-38 B9 and uc.12+A-Romas that overexpress uc.12+A stably were formed. Flow cytometry was used to detect cell apoptosis and cycle, in vitro cell counts were performed to compare cell proliferation, and mice bearing tumors subcutaneously were used to measure tumor weight. The results showed that the expression level of uc.12+A in mouse B lymphoma cells was higher than that of normal mouse B cells. This study suggests that overexpression of uc.12+A significantly promotes the proliferation of B lymphoma cells, reduces their apoptosis, and increases the tumorigenesis rate of B cell lymphoma.

关 键 词：超保守RNA B淋巴瘤细胞 细胞增殖 凋亡 

分 类 号：R733.4[医药卫生—肿瘤]