Anti-inflammatory Activity and Mechanism of Total Flavonoids from the Phloem of Paulownia elongate S.Y. Hu in LPS-stimulated RAW264.7 Macrophages  

作　　者：Liyan LI Tao HUANG Chong LAN 

机构地区：[1]Medical School,Huanghe Science&Technology University,Zhengzhou 450063,China [2]Zhengzhou Key Laboratory of Drug Screening and Activity Evaluation,Huanghe Science&Technology University,Zhengzhou 450063,China

出　　处：《Agricultural Biotechnology》2021年第3期117-120,124,共5页农业生物技术（英文版）

基　　金：Henan Provincial Department of Science&Technology(172102310165);Henan Provincial Department of Education(21B350001).

摘　　要：The juice leaked from broken phloem of Paulownia elongate S.Y.Hu has been used to cure acute inflammation resulted from stung injury by poisonous insects for a long time in China,but its potential mechanism remains unclear.The present study was designed to evaluate anti-inflammatory activity and mechanism of total flavonoids from the phloem of P.elongate S.Y.Hu in RAW264.7 cells.Lipopolysaccharide(LPS)-induced nitric oxide(NO)was measured by Griess and mRNA of pro-inflammatory mediators was analyzed by q-PCR.Cell viability was measured using cell counting kit(CCK)-8 assay.The protein level was analyzed by Western blot.The results showed that the total flavonoids of P.elongate significantly inhibited the production of the pro-inflammatory mediators such as NO,interleukin(IL)-1β and interleukin(IL)-6 in LPS-stimulated RAW264.7 cells.Total flavonoids of P.elongate exerted potential anti-inflammatory activity through the regulation of several signaling pathways.Total flavonoids of P.elongate inhibited JAK/STAT by blocking JAK2 and STAT3 phosphorylation levels.This is the first report on anti-inflammatory activity of total flavonoids from the phloem of P.elongate,which suggests that the total flavonoids of P.elongate may have great potential for the development of anti-inflammatory drug to treat inflammatory disorders.

关 键 词：Paulownia elongate Total flavonoids Anti-inflammatory activity MECHANISMS 

分 类 号：R285[医药卫生—中药学]