miR-149-5p通过HMGA2调控骨肉瘤细胞迁移  

miR-149-5p regulates the migration of osteosarcoma cells through HMGA2

作　　者：朱英会 仲伟俍 王志坚 仲涛 田康[2] ZHU Yinghui;ZHONG Weiliang;WANG Zhijian;ZHONG Tao;TIAN Kang(Department of Orthopedics,The Third People’s Hospital of Dalian,Dalian Medical University,Dalian 116033,China;Department of Orthopedics,The First Affiliated Hospital of Dalian Medical University,Dalian 116011,China)

机构地区：[1]大连医科大学附属大连市第三人民医院骨科,辽宁大连116033 [2]大连医科大学附属第一医院骨科,辽宁大连116011

出　　处：《中国医科大学学报》2021年第6期521-525,共5页Journal of China Medical University

基　　金：辽宁省高等学校科学研究一般项目(L2014360)。

摘　　要：目的探讨miR-149-5p对骨肉瘤(OS)细胞迁移能力的影响。方法通过转染技术构建miR-149-5p过表达细胞系,采用PCR检测OS细胞中miR-149-5p的表达水平,采用Western blotting检测高迁移率族蛋白A2(HMGA2)的表达,采用CCK-8实验检测不同处理因素下OS细胞的迁移能力。通过生物信息学网站starBase结合预测miR-149-5p与HMGA2 mRNA的结合位点。结果与正常细胞相比,OS细胞中miR-149-5p呈低表达趋势(P<0.05),HMGA2呈高表达趋势(P<0.05)。miR-149-5p抑制细胞迁移,并可被HMGA2过表达所恢复。生物信息学网站证明HMGA2 mRNA存在与miR-149-5p的结合位点。结论miR-149-5p通过HMGA2调控OS细胞的迁移。Objective To explore the effect of miR-149-5p on the migration of osteosarcoma cells.Methods The expression of miR-149-5p in osteosarcoma cells was detected using PCR.An miR-149-5p-overexpressing cell line was constructed using transfection technology.The expression of miR-149-5p was detected using PCR,and the expression of high mobility group protein A2(HMGA2)was detected through Western blotting.The migration of cells under different conditions was detected using the Transwell assay.The binding sites of miR-149-5p and the HMGA2 mRNA were predicted through the bioinformatic website StarBase.Results Compared to normal cells,osteosarcoma cells exhibited a lower expression of miR-149-5p(P<0.05)and higher expression of HMGA2(P<0.05).miR-149-5p inhibited cell migration,which could be restored by HMGA2 overexpression.The bioinformatic data revealed that the HMGA2 mRNA can bind to miR-149-5p.Conclusion miR-149-5p regulates the migration of osteosarcoma cells through HMGA2.

关 键 词：miR-149-5p 高迁移率族蛋白A2 迁移 骨肉瘤 

分 类 号：R738.1[医药卫生—肿瘤]