用于食品中玉米赤霉烯酮高灵敏、快速检测的倏逝波免疫传感技术  

Rapid and sensitive detection of ZEN in cereal using an evanescent wave optofluidic immunosensor

作　　者：刘艳萍 方顺燕 徐文娟 刘佳瑶 宋丹[1] 王宏亮 韩向峙 龙峰[1] Yanping LIU;Shunyan FANG;Wenjuan XU;Jiayao LIU;Dan SONG;Hongliang WANG;Xiangzhi HAN;Feng LONG(School of Environment and Natural Resources,Renmin University of China,Beijing,100872,China)

机构地区：[1]中国人民大学环境学院,北京100872

出　　处：《环境化学》2021年第5期1583-1591,共9页Environmental Chemistry

基　　金：中央高校建设世界一流大学(学科)和特色发展引导专项资金资助.

摘　　要：玉米赤霉烯酮(zearalenone,ZEN)是一种由镰刀菌属产生的次级代谢产物,利用课题组自主研发的倏逝波光纤免疫传感器,以玉米赤霉烯酮包被抗原修饰的光纤探头作为生物识别元件,基于间接竞争免疫分析原理,建立了ZEN的高灵敏、快速定量检测方法。在最佳检测条件下,ZEN的检测限为0.35μg·L^(−1),线性区间为0.75—7.48μg·L^(−1),单个检测周期只需8 min。包被抗原修饰的光纤探头可重复使用100次以上而没有明显的性能损失,可以保证检测结果的准确性并降低成本。对小麦、玉米、猪饲料进行了不同浓度的加标回收测试,结果显示加标回收率在80.86%—113.92%范围内,相对标准偏差小于7.90%,表明该传感器具有很好的稳定性和精确度,可以用于食品中ZEN的快速检测,为食品安全评价提供有力分析工具。Zearalenone(ZEN)is a secondary metabolite produced by Fusarium.Based on the indirect competitive immunoassay principle,an portable and reusable evanescent wave optofluidic immunosensor is at the first time reported for rapid and sensitive detection of ZEN using zearalenone antigen modified fiber optic bio-probe as a biological recognition element.Under optimal conditions,the detection limit of ZEN is 0.35μg·L^(−1),and the linear range is 0.75—7.48μg·L^(−1),one detection cycle is within 8 min.The antigen modified fiber optic bio-probe can be reused more than 100 times without obvious performance loss,which ensures the accuracy of the detection results and reduce cost.The recovery of wheat,corn and pig feed with different concentrations was tested.The results showed that the recovery rate was in the range of 80.86%—113.92%,the relative standard deviation is less than 7.90%.These results indicated that this immunosensor has good stability and accuracy and can be employed for the rapid detection of ZEN in food and provides a powerful analysis tool for food safety evaluation.

关 键 词：玉米赤霉烯酮 倏逝波 传感器 检测 

分 类 号：TS207.5[轻工技术与工程—食品科学]