Alcaligenes faecalis WT14的异养硝化-好氧反硝化特性及对高氨废水处理潜力  

HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFICATION CHARACTERISTICS OF ALCALIGENES FAECALIS WT14 AND ITS POTENTIAL APPLICATION IN AMMONIA-RICH WASTEWATER

作　　者：陈均利 张苗苗[1] 张树楠[1] 肖润林[1] 吴金水[1,2] 刘锋 CHEN Jun-li;ZHANG Miao-miao;ZHANG Shu-nan;XIAO Run-lin;WU Jin-shui;LIU Feng(Key Laboratory of Agro-ecological Processes in Subtropical Regions,Changsha Research Station for Agricultural and Environmental Monitoring,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验长沙农业环境观测研究站,长沙410125 [2]中国科学院大学,北京100049

出　　处：《环境工程》2021年第2期27-32,40,共7页Environmental Engineering

基　　金：中科院前沿科学重点研究计划项目(QYZDJ-SSW-DQC041);国家自然科学基金青年科学基金(Y811461);国家自然科学基金项目(41807056、41771302);国家重点研发计划项目(2018YFD0800504)。

摘　　要：异养硝化-好氧反硝化(HN-AD)菌具有同步硝化反硝化的特性,在污水生物处理方面引起了广泛关注。为探究HN-AD菌在处理高氨废水方面的应用潜力,以前期从人工湿地底泥基质中分离出的HN-AD菌Alcaligenes faecalis WT14为研究对象,在好氧条件下研究了该菌株的脱氮特性,并对其高氨废水去除潜力和环境因子交互作用对其脱氮效率影响方面进行了评估。实验结果表明,WT14表现出高效的HN-AD性能,能够同步脱氮除碳,在好氧条件下能够去除98.9%的NO_(3)^(-)-N和92.7%的TOC,且不含NO_(2)^(-)-N的积累。同时,在游离氨(FA)浓度为1084.6 mg/L的高NH_(4)^(+)-N(2000 mg/L)废水中,NH_(4)^(+)-N去除率约为43%,脱氮速率可达55.9 mg/(L·h),且无NO_(3)^(-)-N的积累。响应面结果表明:其最佳脱氮条件为20.6℃,113.5 r/min,C/N=10.8,p H=8.4。总体而言,菌株WT14具有高效处理高氨废水的应用潜力。The ability of HN-AD bacteria to simultaneous nitrification and denitrification caused widespread concern in biological wastewater treatment.In order to investigate the application potential of HN-AD bacteria in ammonia-rich wastewater,the HN-AD bacteria Alcaligenes faecalis WT14 was isolated from the substrate of constructed wetland sediment.The nitrogen removal characteristics of this strain were studied under aerobic conditions,and the effect of the interaction between the removal potential of high ammonia wastewater and environmental factors on its nitrogen removal efficiency was evaluated.The strain WT14 exhibited excellent HN-AD performance and high ammonia tolerance.It could remove 98.9%of NO_(3)^(-)-N and 92.7%of TOC under aerobic conditions,and no NO_(2)^(-)-N accumulation took place in the whole course.At the same time,the strain WT14 treated high ammonia(2000 mg/L)wastewater with a free ammonia concentration of 1084.6 mg/L and removed about 43%NH_(4)^(+)-N without accumulation of NO_(3)^(-)-N.The results of response surface methodology showed that the optimal conditions for denitrification were as follows:20.6℃,113.5 r/min,C/N of 10.8,and p H of 8.4.Overall,the strain WT14 has good potential for ammonia-rich wastewater treatment.

关 键 词：异养硝化-好氧反硝化 同步脱氮 Alcaligenes faecalis 游离氨 氨氮 

分 类 号：X172[环境科学与工程—环境科学] X703