Formative pluripotent stem cells show features of epiblast cells poised for gastrulation  

作　　者：Xiaoxiao Wang Yunlong Xiang Yang Yu Ran Wang Yu Zhang Qianhua Xu Hao Sun Zhen-Ao Zhao Xiangxiang Jiang Xiaoqing Wang Xukun Lu Dandan Qin Yujun Quan Jiaqi Zhang Ng Shyh-Chang Hongmei Wang Naihe Jing Wei Xie Lei Li 

机构地区：[1]State Key Laboratory of Stem Cell and Reproductive Biology,Innovation Academy for Stem Cell and Regeneration,Beijing Institute for Stem Cell and Regenerative Medicine,Institute of Zoology,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Beijing 100101,China [2]Department of Cell Biology and Genetics,School of Basic Medical Sciences,Chongqing Medical University,Chongqing 400016,China [3]State Key Laboratory of Cell Biology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China [4]Center for Stem Cell Biology and Regenerative Medicine,MOE Key Laboratory of Bioinformatics,THU-PKU Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,China [5]Guangzhou Regenerative Medicine and Health Guangdong Laboratory(GRMH-GDL),Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,Guangdong 510530,China

出　　处：《Cell Research》2021年第5期526-541,共16页细胞研究（英文版）

基　　金：The study was supported by the National Key R&D Program of China(2018YFC1004500,2017YFC1001401,2019YFA0508900);the National Natural Science Foundation of China(31930033,31590832,31988101,31725018).

摘　　要：The pluripotency of mammalian early and late epiblast could be recapitulated by naïve embryonic stem cells(ESCs)and primed epiblast stem cells(EpiSCs),respectively.However,these two states of pluripotency may not be sufficient to reflect the full complexity and developmental potency of the epiblast during mammalian early development.Here we report the establishment of self-renewing formative pluripotent stem cells(fPSCs)which manifest features of epiblast cells poised for gastrulation.fPSCs can be established from different mouse ESCs,pre-/early-gastrula epiblasts and induced PSCs.Similar to pre-/early-gastrula epiblasts,fPSCs show the transcriptomic features of formative pluripotency,which are distinct from naïve ESCs and primed EpiSCs.fPSCs show the unique epigenetic states of E6.5 epiblast,including the super-bivalency of a large set of developmental genes.Just like epiblast cells immediately before gastrulation,fPSCs can efficiently differentiate into three germ layers and primordial germ cells(PGCs)in vitro.Thus,fPSCs highlight the feasibility of using PSCs to explore the development of mammalian epiblast.

关 键 词：PLURIPOTENT FEATURES development. 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]