Feasibility study on the use of near-infrared spectroscopy for rapid and nondestructive determination of gossypol content in intact cottonseeds  

作　　者：LI Cheng SU Bangsong ZHAO Tianlun LI Cong CHEN Jinhong ZHU Shuijin 

机构地区：[1]Hainan Institute,Zhejiang University,Sanya,572025,China [2]Lingnan Guangdong Laboratory of Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Science,Shenzhen,518124,Guangdong,China [3]Department of Agronomy,Zhejiang Key Laboratory of Crop Germplasm,Zhejiang University,Hangzhou,310058,China

出　　处：《Journal of Cotton Research》2021年第2期138-146,共9页棉花研究（英文）

基　　金：The research work was funded by The National Key Technology R&D Program of China(2016YFD0101404);China Agriculture Research System(CARS-18-25);Jiangsu Collaborative Innovation Center for Modern Crop Production.

摘　　要：Background:Gossypol found in cottonseeds is toxic to human beings and monogastric animals and is a primary parameter for the integrated utilization of cottonseed products.It is usually determined by the techniques relied on complex pretreatment procedures and the samples after determination cannot be used in the breeding program,so it is of great importance to predict the gossypol content in cottonseeds rapidly and nondestructively to substitute the traditional analytical method.Results:Gossypol content in cottonseeds was investigated by near-infrared spectroscopy(NIRS)and high-performance liquid chromatography(HPLC).Partial least squares regression,combined with spectral pretreatment methods including Savitzky-Golay smoothing,standard normal variate,multiplicative scatter correction,and first derivate were tested for optimizing the calibration models.NIRS technique was efficient in predicting gossypol content in intact cottonseeds,as revealed by the root-mean-square error of cross-validation(RMSECV),root-mean-square error of prediction(RMSEP),coefficient for determination of prediction(R_(p)^(2)),and residual predictive deviation(RPD)values for all models,being 0.05∼0.07,0.04∼0.06,0.82∼0.92,and 2.3∼3.4,respectively.The optimized model pretreated by Savitzky-Golay smoothing+standard normal variate+first derivate resulted in a good determination of gossypol content in intact cottonseeds.Conclusions:Near-infrared spectroscopy coupled with different spectral pretreatments and partial least squares(PLS)regression has exhibited the feasibility in predicting gossypol content in intact cottonseeds,rapidly and non destructively.It could be used as an alternative method to substitute for traditional one to determi ne the gossypol content in intact cottonseeds.

关 键 词：Intact cottonseed CHEMOMETRICS GOSSYPOL Near-infrared spectroscopy 

分 类 号：O657.33[理学—分析化学] TS119[理学—化学]