SMURF2在前列腺癌细胞中调节STAT1的泛素化并促进EMT  

SMURF2 Regulates STAT1 Ubiquitination and Promotes EMT in Prostate Cancer Cells

作　　者：董佳杰 党燕梅 张驰 蒋顺 刘平[1] 卢山[1] Dong Jiajie;Dang Yanmei;Zhang Chi;Jiang Shun;Liu Ping;Lu Shan(School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Nanjing 210023,China)

机构地区：[1]南京师范大学生命科学学院,生物化学与生物制品研究所,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2021年第2期62-68,共7页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(81272850、81472415).

摘　　要：SMURF2(smad ubiquitination regulatory factor 2)属于HECT(homologous to E6AP C terminus)家族E3泛素连接酶,有研究报道SMURF2在不同类型癌症中发挥促癌或抑癌作用.本研究探讨了SMURF2在前列腺(癌)细胞中调节STAT1(signal transducers and activators of transcription 1)蛋白的分子机制.首先通过数据库分析SMURF2在正常组织与肿瘤组织间的表达差异,然后选取前列腺正常细胞和多种前列腺癌细胞为实验材料,通过RT-PCR,Western blotting实验检测SMURF2在前列腺(癌)细胞中的表达水平,发现相对于前列腺正常细胞,SMURF2在前列腺癌细胞中表达更高.再通过Co-IP,免疫荧光和泛素化检测实验观察SMURF2对STAT1蛋白泛素化水平的影响,发现SMURF2可以增加STAT1蛋白的泛素化水平,并进一步促进前列腺癌细胞发生EMT(epithelial mesenchymal transformation).SMURF2(smad ubiquitination regulatory factor 2)is a HECT(homologous to E6AP C terminus)family E3 ubiquitin ligase.Studies have shown that SMURF2 plays a role in promoting or suppressing cancer progression in different types of cancer.This study explored the molecular mechanism of SMURF2 regulating STAT1(signal transducers and activators of transcription 1)protein in prostate(cancer)cells.The database was firstly used to analyze the expression level of SMURF2 in different normal tissues and tumor tissues.Normal prostate cells and various prostate cancer cells were selected as experimental materials.The expression level of SMURF2 in prostate(cancer)cells was detected by RT-PCR and Western blotting assays,which found that the expression level of SMURF2 was higher in prostate cancer cells than normal prostate cells.Then the Co-IP,immunofluorescence and ubiquitination testing experiments were used to explore the effects on SMURF2 on the ubiquitination of STAT1 protein level,which found that SMURF2 can increase the ubiquitination level of STAT1 protein and further promote EMT(epithelial mesenchymal transformation)in prostate cancer cells.

关 键 词：前列腺癌 SMURF2 STAT1 EMT 

分 类 号：Q291[生物学—细胞生物学]