采用Box-Benhnken响应面法优化致命鹅膏α-鹅膏毒肽超声波提取工艺  

Optimization of Ultrasonic Extraction ofα-Amanitin from Amanita exitialis by Box-Benhnken Response Surface Design

作　　者：刘萌 甘莉霞 张林平[1] 李泰辉 邓旺秋 LIU Meng;GAN Lixia;ZHANG Linping;LI Taihui;DENG Wangqiu(Key Laboratory of National Forestry and Grassland Administration on Forest Ecosystem Protection and Restoration of Poyang Lake Watershed,College of Art and Landscape,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;Institute of Microbiology,Guangdong Academy of Sciences,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,Guangdong,China)

机构地区：[1]江西农业大学林学院,鄱阳湖流域森林生态系统保护与修复国家林业和草原局重点实验室,江西南昌330045 [2]广东省科学院微生物研究所,华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《食用菌学报》2021年第3期93-101,共9页Acta Edulis Fungi

基　　金：广州市科技计划重点项目(201804020018);国家自然科学基金(31970024);广东省科技计划(2019B121202005、2018B020205001)。

摘　　要：通过单因素实验考察提取次数、超声波处理时间、甲醇体积浓度、超声波功率和料液比对致命鹅膏(Amanita exitialis)α-鹅膏毒肽提取得率的影响,获得关键因素及其最佳条件,再采用Box-Benhnken响应面法优化α-鹅膏毒肽超声波提取工艺。研究结果表明:致命鹅膏α-鹅膏毒肽最佳提取工艺为提取2次、超声波处理15 min、甲醇体积浓度35%、超声波功率300 W、料液比1∶20(g∶mL),α-鹅膏毒肽提取得率为3.33 mg·g^(-1)干重,与预测值3.30 mg·g^(-1)干重无显著差异(P>0.05),说明获得的最佳提取工艺稳定、可行。Using single-factor experiments,ultrasonic treatment time,volumetric methanol concentration,ultrasonic power,material to liquid ratio,and number of repeats of the extraction were studied for their effects on Amanita exitialisα-amanitin yield so as to obtain an optimal condition for each key extraction factor.Then the Box-Benhnken response surface method was used to optimize the ultrasonic extraction process.The results showed that the optimal extraction process for A.exitialisα-amanitin was as follows:ultrasonic treatment time of 15 min,35%(V∶V)methanol,300 W ultrasonic power,material-to-liquid ratio of 1∶20(g∶mL),and 2 times of repeats of the extraction.Using the optimal process,the peptide yield was 3.33 mg·g^(-1)dry weight,which was close to the predicted value of 3.30 mg·g^(-1)dry weight(P>0.05).This optimal extraction process is stable and feasible.

关 键 词：致命鹅膏 α-鹅膏毒肽 得率 响应面 

分 类 号：TQ28[化学工程—有机化工]